Photopatterning with PRIMO

CAN PRIMO BE USED TO PATTERN PROTEINS ON 3D STRUCTURES?

PRIMO can be used to pattern all surfaces, whether flat, curved or microstructured. For example, it is possible to pattern proteins on microwell walls or PDMS micropillars. In-house data available on request.
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PRIMO micropatterning for cryo-ET

What difference does it makes to use PRIMO to prepare my cell samples for cryo-ET ?

PRIMO maskless micropatterning system allows to control cell adhesion. Therefore, it guarantees you will get several cells positioned precisely where you want them to be on the EM grid (usually in the middle of the square composing the mesh of the grid). Futhermore, it gives you full control over the design of micropattern you create. As shown in some of our users research works, you can then control cell shapes, polarity, spreading, and that for:
  • mechanobiology studies
  • facilitating the FIB milling process
  • ensuring reproducible and more efficient cryo-ET imaging
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The PRIMO module

WHAT ARE THE DIMENSIONS AND WEIGHT OF THE PRIMO MODULE?

The dimensions of the PRIMO unit are 31 x 24.3 x 7.5 cm (length x width x height). It weighs 5 kg.
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The dedicated Leonardo software

WHAT ARE THE FEATURES OF THE LEONARDO SOFTWARE?

  • Replication of the same pattern (settings in rows and columns, and adjustment of spacing)
  • Realignment of successive patterns or on microstructures (preview, rotation)
  • Gradient management
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Photopatterning platform and reagents

WHAT EQUIPMENT IS NEEDED TO USE PRIMO?

  • Inverted microscope fitted with focus maintaining system(1):
    • Nikon Ti2-E and Ti-E
    • Leica DMi8
    • Olympus IX83
    • Zeiss AxioObserver
  • Epifluorescence system
  • Motorized X/Y stage
  • Camera
  • Computer
  • Plasma reactor and dedicated pump(2)
(1)Full microscope configuration available on request. (2)Optional depending on experimental conditions.
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