During development, neurons migrate in response to gradients of molecules to reach their final destination in the brain.

PRIMO technology offers the possibility to micropattern gradients of molecules and is therefore particularly suitable for quantitatively determining the chemo-attractant or chemo-repellent effect of axon guidance cues.

wheel pattern of laminin
Chicken brain explant positioned in the center of a wheel pattern of laminin labeled with alexa488 (green). Courtesy of H. Ducuing, R. Moore, Y. Lecomte, P.-O. Strale and V. Studer.

Axon guidance on the spokes and around the circumference of the laminin wheel pattern (24-hours time-lapse video). Courtesy of H. Ducuing, R. Moore, Y. Lecomte, P.-O. Strale and V. Studer.

Primary hippocampal neurons on a grid of laminin optimized and patterned with PRIMO.

Nathalie Piette - Neuron micropattern
Epifluorescence image of rat hippocampal neurons (DIV14) immunostained with Anti-SynCAM1 (Secondary antibody coupled to A488) in culture on SynCAM1-Fc micropatterns done with PRIMO (5 µm dots). Courtesy of Nathalie Piette, Cell Organ-izers Joint Lab, IINS Bordeaux and Alvéole.
Micropattern image of dots
5 µm dots micropatterns of SynCAM1-Fc used to generate the neuronal network.

Do you have a question about your project of experiment with PRIMO?

Our research and application development team can help you set up or optimize your experimental protocols!

  • This field is for validation purposes and should be left unchanged.

the cellular

Alvéole has developed innovative solutions adapted to all standard cell culture substrates, rigid or soft, in 2D or 3D.

Learn more

Resource Center

Our team gives you all its tips you need to carry out your experimental manipulations and go even further!

Learn more


Our users describe their research projects and explain why they chose to use PRIMO!

Read the testimonials