Reproducible spheroid formation on protein micropatterns, using PRIMO.

This technique of spheroid formation by cell aggregation on custom micropatterns shows the following advantages:

  • control over cell biochemical microenvironment,
  • compatible with all standard cell culture substrates.
Huh-7 cells forming spheroids on micropatterns of fibrinogen-A488 (micropatterns Ø= 200 μm)
Huh-7 cells forming spheroids on micropatterns of fibrinogen-A488 (wells Ø= 500 μm, patterns Ø= 300 μm)

Homogeneous spheroid formation in hydrogel microwells photopolymerized using PRIMO.

The custom photopolymerization of hydrogels in any shape you want provides you with a precise and reproducible control over the mechanical properties of the cell microenvironment in 3D (shaping, stiffness).

Spheroids of HEK cells in hydrogel microwells photopolymerized with PRIMO. Hydrogel microwells size: ⌀=100 µm, H=175 µm. Courtesy of A. Pasturel and V. Studer (IINS, CNRS)

Spheroids of HEK cells in hydrogel microwells photopolymerized with PRIMO. Hydrogel microwells size: ⌀=200 µm, H=175 µm. Courtesy of A. Pasturel and V. Studer (IINS, CNRS)

Do you have a question about your project of experiment with PRIMO?

Our research and application development team can help you set up or optimize your experimental protocols!

Controlling
the cellular
microenvironment

Alvéole has developed innovative solutions adapted to all standard cell culture substrates, rigid or soft, in 2D or 3D.

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Resource Center

Our team gives you all its tips you need to carry out your experimental manipulations and go even further!

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Testimonials

Our users describe their research projects and explain why they chose to use PRIMO!

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