This year again, our team will be at the ASCB EMBO 2019 annual meeting to present you PRIMO micropatterning system and its applications for cell biology research work.

–> Controlled & reproducible cell microenvironments in vitro.

Applications in cell biology conducted using PRIMO micropatterning

Tech Talk and Discussion

Where lies the added value of Micropatterning, Microfabrication or Hydrogel polymerization for in vitro cell experiments ? Presentation of the latest research works using PRIMO® photopatterning system.

MONDAY 9 DECEMBER, 4:15PM – 5:15PM / Theater 1

In vitro cell experiments confront researchers with many challenges; such as the recurring reproducibility issues, reliability in term of physiological relevance, but also ease of use and efficiency. Here we present three technologies answering those labs’ daily challenges:

(1) MICROPATTERNING allows to precisely control cell adhesion to isolate them or place them in reproducible conditions for standardized assays. Several of our users’ research works show the advantages of PRIMO contactless micropatterning – at single cell, cell population and high throughput levels: Cell positioning within EM grids for Cryo-ET (M. Toro-Nahuelpan et al., BioRxiv, 2019), airway smooth muscle cells patterning on gel for contraction study (S. R. Polio et al., Sci. Rep., 2019).

(2) MICROFABRICATION: One key advantage of PRIMO maskless DMD-based photopatterning system is that it can perform greyscale photolithography on greyscale resists (collaboration with Physico-Chimie Curie UMR 168 and UMS-IPGG) and therefore create complex 3D molds such as ramps, curving wells, microfluidic chips for organ-on-a-chip applications.

(3) HYDROGEL POLYMERIZATION: PRIMO can also polymerize and photo-scission most commonly used hydrogels (A. Pasturel et al., BioRxiv, 2018) for applications such as 3D cell culture within micro-niches (C. Stoecklin et al., Adv. Biosys., 2018) or permeable hydrogel membranes polymerization within microfluidic chips (J. Decock et al., Lab. Chip, 2018).

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