#Cryo-CLEM 2 Hands-on Tutorial: Workflow Advances for Whole Cell Correlative Cryo-Microscopy
CRYO-CLEM WEBINAR SERIES - No2 - Correlative (Cryo-)Microscopy for Cell Biology
Date: July 2, 2020
Speakers: Pr. Elizabeth R. Wright, Dr Jae E. Yang, Dr Bryan S. Sibert and Dr Joseph Y. Kim – from Morgridge Institute for Research University of Wisconsin-Madison (USA)
Correlative light and electron microscopy (CLEM) is used to accelerate biological discoveries. CLEM bridges between different microscopes and imaging modalities to study systems in 4D, effectively spanning gaps between cell and structural biology. CLEM technologies are rapidly advancing in order to improve biological understanding, experimental reproducibility, and automation.
In this 2nd webinar, Pr Wright and her team will expand upon the basics of the CLEM technologies that were presented in the first webinar. They will describe successful step-by-step procedures for optimized cell micropatterning on substrates, cell culture on EM grids and whole-cell cryo-CLEM imaging. They will also present validation steps and timing associated with each step of the optimized workflow. Finally, they will give examples of biological studies conducted using these workflows.
Learning Objectives:
- Selecting necessary materials and instrumentation for cryo-CLEM.
- Optimizing the cryo-CLEM pipeline by integrating micro-patterning methods.
- Understanding workflows for EM grids preparation, cell culture on EM grids, and cryo-CLEM imaging.
This webinar series is presented by Pr Elizabeth Wright and her team from Morgridge Institute for Research University of Wisconsin-Madison (USA). Alvéole is proud to sponsor this series of cryo-CLEM webinars in partnership with Leica Microsystems!