PLPP

The photoactivatable reagent

New PLPP Gel: Accelerate the speed of your protein micropatterning experiments and substrate biofunctionalization up to 30 times compared to PLPP Classic.

High throughput micropatterning

Contrary to traditional photopatterning techniques, PRIMO technology associates a maskless photolithography system, PRIMO, and a specific photoactivatable reagent, PLPP (Classic or Gel). Coupled with UV, the PLPP enables the micropattern to be created on the illuminated area of the substrate.

Photopatterning is a subtractive technology. It first consists in covering a substrate with an anti-fouling polymer to prevent molecules from adhering to it. For “traditional” photopatterning techniques, this anti-fouling surface is then locally degraded using deep UV lights through a mask.

* Based on LIMAP technology protected by two patents filed by the CNRS and the University of Bordeaux.

With PRIMO* photopatterning technology, a UV illumination (λ=375nm) of 500 µm x 300 µm is projected through a microscope objective (20x). Adding PLPP Gel on the substrate before illumination allows the anti-fouling polymer to be degraded in only one second, allowing to engineer hundreds of micropatterns in a few minutes.

Association of the PRIMO photopatterning module and the photoactivatable reagent, PLPP (Classic or Gel)

01 SUBSTRATE PREPARATION: PLPP is added onto a substrate treated with an anti-fouling polymer (PEG).

Alveole-PRIMO

02 IMAGE LOADING IN THE PRIMO SYSTEM: From right to left: Pattern uploading into Leonardo Software. Pattern projection in UV light by PRIMO through the microscope objective onto the substrate.

Alveole-PRIMO

03 UV ILLUMINATION: The PLPP combined with UV degrades the anti-fouling layer.

Alveole-PRIMO

04 LOCAL DEGRADATION OF THE ANTI-FOULING POLYMER: Once the illumination is finished, the anti-fouling polymer has been locally degraded and the PLPP is rinsed.

Alveole-PRIMO

05 DEPOSITION OF PROTEIN: Proteins bind to the illuminated areas only.

Alveole-PRIMO

06 PROTEIN MICROPATTERN: Rinsing of the excess of protein and visualization of the protein micropattern.

Alveole-PRIMO

07 CELL ADHESION: Cells are seeded and adhere to the protein micropattern only.

alveole-primo

New: PLPP Gel protocol of use in video

Specifications

Photo-initiator

λ=375nm

Accelerator

1 sec

for a full field pattern *

*Approx. 500×300µm, 20x objective, with PLPP Gel

Ready to use

in solution

Patterning time
Full field pattern (500 x 300 µm), 20x objective
1 sec (white pattern)

4 sec (gradient)

30 – 40 sec

(white pattern or gradient)

Substrates
Glass
Stiff PDMS (fully reticulated): Flat or Structured
Soft PDMS: Flat or Structured
Transfer on polyacrylamide gel (using a coverslip)
Microfluidic chip
Protocols
Plasma cleaning Mandatory Non-mandatory
PEG SVA
PLL-G-PEG
Objective 20x only 20x or 4x

Resources and Support

Our team gives you all the tips to successfully conduct your experimental manipulations and go even further!

Video
tutorials

Scientific
papers

Application notes

See all resources

For an optimized and personalized control over your experimental conditions, discover complementary PRIMO products.

Request an offer
or a demo
If you would like to test PRIMO on one of your experimental projects, receive a quote or simply to know more about Alvéole and its products

Our team will be happy to answer any questions you may have.

+33 (0)1 84 25 51 41
Send us
your request
  • This field is for validation purposes and should be left unchanged.