The photoactivatable reagent
Coupled with UV, it enables the pattern to be created on the illuminated area of the substrate. You can thus precisely apply the protein of your choice.
Contrary to traditional photopatterning techniques, PRIMO technology associates a maskless photolithography system, PRIMO, and a specific photoactivatable reagent, PLPP.
Photopatterning is a subtractive technology. It first consists in covering a substrate with an anti-fouling polymer to prevent molecules from adhering to it. For “traditional” photopatterning techniques, this anti-fouling surface is then locally degraded using deep UV lights through a mask.
* Based on LIMAP technology protected by two patents filed by the CNRS and the University of Bordeaux.
With PRIMO* photopatterning technology, a UV illumination at a wavelength of 375 nm is projected through a microscope objective. Adding PLPP on the substrate during illumination allows the anti-fouling polymer to be degraded in less than a minute.
01 SUBSTRATE PREPARATION: PLPP is added onto a substrate treated with an anti-fouling polymer (PEG).
02 IMAGE LOADING IN THE PRIMO SYSTEM: From right to left: Pattern uploading into Leonardo Software. Pattern projection in UV light by PRIMO through the microscope objective onto the substrate.
03 UV ILLUMINATION: The PLPP combined with UV degrades the anti-fouling layer.
04 LOCAL DEGRADATION OF THE ANTI-FOULING POLYMER: Once the illumination is finished, the anti-fouling polymer has been locally degraded and the PLPP is rinsed.
05 DEPOSITION OF PROTEIN: Proteins bind to the illuminated areas only.
06 PROTEIN MICROPATTERN: Rinsing of the excess of protein and visualization of the protein micropattern.
07 CELL ADHESION: Cells are seeded and adhere to the protein micropattern only.
for a full field pattern *
*Approximately 500×300µm, 20x objective
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