PLPP

The photoactivatable reagent

PLPP Gel: Accelerate the speed of your protein micropatterning experiments and substrate biofunctionalization up to 30 times compared to PLPP Classic.

High throughput micropatterning

Contrary to traditional photopatterning techniques, PRIMO technology associates a maskless photolithography system, PRIMO, and a specific photoactivatable reagent, PLPP (Classic or Gel). Coupled with UV, the PLPP enables the micropattern to be created on the illuminated area of the substrate.

Photopatterning is a subtractive technology. It first consists in covering a substrate with an anti-fouling polymer to prevent molecules from adhering to it. For “traditional” photopatterning techniques, this anti-fouling surface is then locally degraded using deep UV lights through a mask.

* Based on LIMAP technology protected by two patents filed by the CNRS and the University of Bordeaux.

With PRIMO* photopatterning technology, a UV illumination (λ=365nm) of 500 µm x 300 µm is projected through a microscope objective (20x). Adding PLPP Gel on the substrate before illumination allows the anti-fouling polymer to be degraded in only one second, allowing to engineer hundreds of micropatterns in a few minutes.

Association of the PRIMO photopatterning module and the photoactivatable reagent, PLPP (Classic or Gel)

01 ANTI-FOULING COATING: The surface of the substrate is coated with an anti-fouling polymer (PEG).

Anti-fouling coating before micropatterning with RPIMO

02 PHOTO-INITIATOR: PLPP is added onto the substrate treated with an anti-fouling polymer.

03 IMAGE LOADING IN THE PRIMO SYSTEM: Pattern uploading into Leonardo Software and projection in UV light by PRIMO through the microscope objective onto the substrate.

04 UV ILLUMINATION: The PLPP combined with UV degrades the anti-fouling layer.

05 LOCAL DEGRADATION OF THE ANTI-FOULING POLYMER: After illumination, the anti-fouling polymer has been locally degraded and the PLPP is rinsed.

06 DEPOSITION OF PROTEIN: Proteins bind to the illuminated areas only.

07 PROTEIN MICROPATTERN: Rinsing of the excess of protein and visualization of the protein micropattern.

08 CELL ADHESION: Cells are seeded and adhere to the protein micropattern only.

New: PLPP Gel protocol of use in video

#7 Protein micropatterning with PLPP Gel

Specifications

Photo-initiator

λ=365nm

Accelerator

0.5 sec

for a full field pattern *

*Approx. 500×300µm, 20x objective, with PLPP Gel

Ready to use

in solution


Patterning time
Full field pattern (500 x 300 µm), 20x objective	0.5 sec (white pattern) 4 sec (gradient)	10 – 20 sec (white pattern or gradient)
Substrates
Glass
Stiff PDMS (fully reticulated): Flat or Structured
Soft PDMS: Flat or Structured
Transfer on polyacrylamide gel (using a coverslip)
Microfluidic chip
Protocols
Plasma cleaning	Mandatory	Non-mandatory
PEG SVA
PLL-G-PEG
Objective	20x only	20x or 4x