The photoactivatable reagent

PLPP Gel: Accelerate the speed of your protein micropatterning experiments and substrate biofunctionalization up to 30 times compared to PLPP Classic.

High throughput micropatterning

Contrary to traditional photopatterning techniques, PRIMO technology associates a maskless photolithography system, PRIMO, and a specific photoactivatable reagent, PLPP (Classic or Gel). Coupled with UV, the PLPP enables the micropattern to be created on the illuminated area of the substrate.

Photopatterning is a subtractive technology. It first consists in covering a substrate with an anti-fouling polymer to prevent molecules from adhering to it. For “traditional” photopatterning techniques, this anti-fouling surface is then locally degraded using deep UV lights through a mask.

* Based on LIMAP technology protected by two patents filed by the CNRS and the University of Bordeaux.

With PRIMO* photopatterning technology, a UV illumination (λ=365nm) of 500 µm x 300 µm is projected through a microscope objective (20x). Adding PLPP Gel on the substrate before illumination allows the anti-fouling polymer to be degraded in only one second, allowing to engineer hundreds of micropatterns in a few minutes.

Association of the PRIMO photopatterning module and the photoactivatable reagent, PLPP (Classic or Gel)

01 ANTI-FOULING COATING: The surface of the substrate is coated with an anti-fouling polymer (PEG).

Anti-fouling coating before micropatterning with RPIMO

02 PHOTO-INITIATOR: PLPP is added onto the substrate treated with an anti-fouling polymer.


03 IMAGE LOADING IN THE PRIMO SYSTEM: Pattern uploading into Leonardo Software and projection in UV light by PRIMO through the microscope objective onto the substrate.


04 UV ILLUMINATION: The PLPP combined with UV degrades the anti-fouling layer.


05 LOCAL DEGRADATION OF THE ANTI-FOULING POLYMER: After illumination, the anti-fouling polymer has been locally degraded and the PLPP is rinsed.


06 DEPOSITION OF PROTEIN: Proteins bind to the illuminated areas only.


07 PROTEIN MICROPATTERN: Rinsing of the excess of protein and visualization of the protein micropattern.


08 CELL ADHESION: Cells are seeded and adhere to the protein micropattern only.


New: PLPP Gel protocol of use in video





0.5 sec

for a full field pattern *

*Approx. 500×300µm, 20x objective, with PLPP Gel

Ready to use

in solution

Patterning time
Full field pattern (500 x 300 µm), 20x objective
0.5 sec (white pattern)

4 sec (gradient)

10 – 20 sec

(white pattern or gradient)

Stiff PDMS (fully reticulated): Flat or Structured
Soft PDMS: Flat or Structured
Transfer on polyacrylamide gel (using a coverslip)
Microfluidic chip
Plasma cleaning Mandatory Non-mandatory
Objective 20x only 20x or 4x

Resources and Support

Our team gives you all the tips to successfully conduct your experimental manipulations and go even further!



Application notes

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For an optimized and personalized control over your experimental conditions, discover complementary PRIMO products.

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