The photoactivatable reagent
PLPP Gel: Accelerate the speed of your protein micropatterning experiments and substrate biofunctionalization up to 30 times compared to PLPP Classic.
Contrary to traditional photopatterning techniques, PRIMO technology associates a maskless photolithography system, PRIMO, and a specific photoactivatable reagent, PLPP (Classic or Gel). Coupled with UV, the PLPP enables the micropattern to be created on the illuminated area of the substrate.
Photopatterning is a subtractive technology. It first consists in covering a substrate with an anti-fouling polymer to prevent molecules from adhering to it. For “traditional” photopatterning techniques, this anti-fouling surface is then locally degraded using deep UV lights through a mask.
* Based on LIMAP technology protected by two patents filed by the CNRS and the University of Bordeaux.
With PRIMO* photopatterning technology, a UV illumination (λ=365nm) of 500 µm x 300 µm is projected through a microscope objective (20x). Adding PLPP Gel on the substrate before illumination allows the anti-fouling polymer to be degraded in only one second, allowing to engineer hundreds of micropatterns in a few minutes.
01 ANTI-FOULING COATING: The surface of the substrate is coated with an anti-fouling polymer (PEG).
02 PHOTO-INITIATOR: PLPP is added onto the substrate treated with an anti-fouling polymer.
03 IMAGE LOADING IN THE PRIMO SYSTEM: Pattern uploading into Leonardo Software and projection in UV light by PRIMO through the microscope objective onto the substrate.
04 UV ILLUMINATION: The PLPP combined with UV degrades the anti-fouling layer.
05 LOCAL DEGRADATION OF THE ANTI-FOULING POLYMER: After illumination, the anti-fouling polymer has been locally degraded and the PLPP is rinsed.
06 DEPOSITION OF PROTEIN: Proteins bind to the illuminated areas only.
07 PROTEIN MICROPATTERN: Rinsing of the excess of protein and visualization of the protein micropattern.
08 CELL ADHESION: Cells are seeded and adhere to the protein micropattern only.
for a full field pattern *
*Approx. 500×300µm, 20x objective, with PLPP Gel
|Full field pattern (500 x 300 µm), 20x objective
||0.5 sec (white pattern)
4 sec (gradient)
|10 – 20 sec
(white pattern or gradient)
|Stiff PDMS (fully reticulated): Flat or Structured|
|Soft PDMS: Flat or Structured|
|Transfer on polyacrylamide gel (using a coverslip)|
|Objective||20x only||20x or 4x|
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