Your project of experiment with PRIMO

“My research focuses on the mechanical aspects of cell division. With the PRIMO system we wanted to control cellular geometry to impose spatial constraints during mitotic progression.”

HeLa-cells-micropatterns
HeLa cell expressing LifeAct-mCherry and EB3-GFP on a fluorescent fibrinogen-Alexa647 micropattern.
HeLa-cells-micropattern
HeLa cells expressing LifeAct-mCherry and EB3-GFP on a fluorescent fibrinogen-Alexa647 micropattern.

Why PRIMO

We have used micropatterning extensively in the past, namely a deep-UV based micropatterning setup. The main issues with this setup have to do with pattern reproducibility and also the costs associated with quartz mask design.

PRIMO was initially recommended by one of our collaborators (M. Piel, Institut Curie).

Little extra which makes all the difference

Performances :

Overall, we were satisfied with the performance of the PRIMO system, although there is a lot of optimization when we first learn how to use the setup.

That being said, once the initial obstacles are surpassed, it then becomes very easy to test a wide range of conditions and quickly define which is the best design.

Moreover, the fact that we can use the same system to generate multiprotein patterns and even create 3D structures makes it a very powerful setup.

If I were to describe PRIMO in one word I would choose “flexibility”.

Support provided by Alvéole team:

My overall experience with the team was very positive. They were always available and helpful when troubleshooting.

“We are working on the generation of 3D cellular microenvironments to reproduce Hematopoietic Niches. PRIMO will be used to generate 3D photo-polymerized microenvironments and to pattern them to localize different cell populations involved in the hematopoiesis.”

Read the testimonial

“We are currently particularly interested in determining the role of the biophysical environment in the establishment of apico-basal polarity in mammary gland cells and in liver cells. The use of PRIMO in this context proved absolutely essential since it allowed us to create artificial microniches in 3D where we could control up to 150 combinations of environmental cues.”

Read the testimonial

“Our aim is to develop in vitro experimentation to decipher guiding mechanisms involved in vivo. PRIMO technology is particularly adapted to design in vitro microdevices patterned with controlled patches of the signaling proteins relevant for white blood cell migration.”

Read the testimonial

“We are interested in imaging subcellular localization of certain cell-surface receptors and check whether they colocalize with focal-adhesion complexes. For this purpose, we are interested in making different types of patterns of Fibronectin with subcellular dimensions.”

Read the testimonial

“My research project aims at unravelling how a T cell switches from a fast migratory state to a stationary state upon activation. To do so, I perform live cell imaging of T cells migrating inside micro-fabricated channels coated with activating molecules. However, with this approach, I do not control when and where a T cell encounters the activating molecules.”

Read the testimonial

“Protein micropatterning represents an excellent tool to probe the behavior and functions of cellular systems. PRIMO is specially suited for our experiments, in which the cell-substrate interaction needs to be precisely adjusted both throughout the substrates and in time, in order to control the dynamic behaviour of cell monolayers.”

Read the testimonial

Controlling
the cellular
microenvironment

Alvéole has developed innovative solutions adapted to all standard cell culture substrates, rigid or soft, in 2D or 3D.

Learn more

Resource Center

Our team gives you all its tips you need to carry out your experimental manipulations and go even further!

Learn more

Testimonials

Our users describe their research projects and explain why they chose to use PRIMO!

Read the testimonials