Leonardo software automatically detects structures on your substrates, such as the mesh of a TEM Grid, and then positions your micropatterns within these microstructures while leaving you free to tune their sizes.
Leonardo proposes two ways to generate series of homogeneous micropatterns automatically positioned within the mesh of the TEM Grid, without damaging its surface (contactless technique):
In the end, PRIMO contactless micropatterning system allows to make sure cells are well positioned and confined within the grids for imaging them in reproducible conditions, as shown by L. Engel et al.* and by M.
* L. Engel et al., Journal of Micromechanics and Microengineering, 2019
** M.