Unrivalled performances

  • Micropatterns alignment with preexisting 2.5D topographies
  • Micropatterns of biomolecules in 3D environment
  • Ease studies on cellular responses to different topological cues


Micropatterns alignment with preexisting 2.5D topographies

PRIMO has a unique alignment capability to precisely position micropatterns of biomolecules on microstructures generating biofunctionalized substrates which are keys to the understanding of cellular processes.

Down below, a wide range of images were designed and loaded into Leonardo software to precisely position and align the different patterns on the microstructures under the microscope.

A: Screenshot of Leonardo software showing the alignment of pattern previews onto structures. B: Fluorescent fibronectin micropatterns (red) onto structures. C: Epifluorescence image of MEF cells stained for actin (green) and nucleus (blue) into patterned micro-wells (top) and onto micro-pillars (bottom). App Note, 2018.

The next picture is an example of three hepatocytes that adhered on fibronectin printed in 3D to determine the minimal distance between hepatocytes to create a biliary canaliculi.

3 hepatocytes HepG2 adhering on patterns of fibronectin on the sides and the bottom of a micro-well. Courtesy of C. Stoecklin and V. Viasnoff.

Micropattern your biomolecule of interest in 3D

With PRIMO you can easily direct the 3D organization of single cells or multicellular arrangements by controlling cell confinement.

In this example, single cells are seeded into micropatterned microwells and matched the 3D micropatterns. This experiment helped the comparison of nucleus x,y,z localisation in between the different geometrical shapes of the cells.

Human Foreskin Fibroblasts adhering on Fibronectin Rhodamin patterns projected on the bottom and the wall of the microwells (60 μm diameter and 30 μm height), Stoecklin et al., Adv. Biosys. 2018.

Ease studies on cellular responses to different topological cues

The PRIMO technology allows a rapid screening of size and a precise pattern alignment to study cellular response to multiple microenvironmental cues.

In the following paper, they studied the impact of micropatterned convex and concave 2.5 D structures on cell guidance. PRIMO enables patterning of extracellular proteins on a 2.5D cell culture chip containing well-defined microstructures that mimic the in-vivo cellular environment.

Outline of the experimental procedure of patterning on structured cell culture substrates.
(B) Example outlines of single hmFBs on patterned curvatures used for quantification of the orientation response. The direction of the contact-guidance cues and curvature is indicated with red bars. (C) Orientation response of hmFBs on both flat and structured PDMS C.van der Putten et al., ACS Publications, 2021.

Here, they studied the role of the extracellular matrix in cell morphology by patterning ECM proteins on nanotopographies to observe influence of those chemical and mechanical cues on the cell organization.

Structural impact of nanotopographical patterned substrates, Hamed Ghazizadeh et al., Nanosurface Biomedical, 2018.

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