Unrivalled performances

  • Tune pattern geometries to impose different cell shapes to study intracellular cytoskeleton organization
  • Induce subcellular events such as focal adhesion dynamics by a micron scale protein micropatterning
  • Change cell confinement to elucidate intrinsic cellular forces

Tune pattern geometries

PRIMO allows a precise control of the cell adhesion to elucidate its impact on the intracellular cytoskeleton organization of a single cell. This makes it a perfect tool to study isolated cells under reproducible conditions.

Examples of cells plated on adhesive patches. Alessandro Dema et al., Methods in Molecular Biology, 2022
Embryonic fibroblasts from vimentin knockout mice on fibronectin + fibrinogen-A647 (blue) patterns of different shapes, actin labeled with phalloidin-A555 (red) and focal adhesions revealed via Anti-Paxillin antibodies + secondary antibodies coupled to A488 (green). Courtesy of A.J. Jimenez and B. Vianay, Physics of cytoskeleton & Morphogenesis lab.
Induce subcellular events

With a resolution down to 1,2 µm, dynamic events which happen in the cell can be observed precisely. Down below, subcellular sized patterns realized with PRIMO to understand the interrelation between microtubules and actin cytoskeleton in the mecanoregulation of integrin-mediated adhesions.

HT1080 cell depleted of KANK1 and KANK2 adhering on small round patterns, focal adhesion labeled with GFP-vinculin (red) and microtubule plus tips labeled with mKO-EB3 (green). The dynamic of microtubule polymerization is shown on the right image. Scale bar, 10 μm. Mohd Rafiq, Y. Nishimura et al., BioRxiv, 2018

KANK proteins are required for targeting microtubules to focal adhesions. KANK1 and 2 knockdown cells showing microtubules
(α-tubulin) and focal adhesions (paxillin).Scale bars, 10 μm. Mohd Rafiq, Y. Nishimura et al., BioRxiv, 2018.

Change cell confinement to elucidate intrisic cellular forces 

Here, they wanted to demonstrate how localized modulation of cell rheology governs cell-cell contact. The geometric constraints impose different cortical stiffness in single, doublets or triplets of cells.

Geometric constraints impose different cortical stiffness in cell pairs.K. Sri-Ranjan et al., Nature Communications, 2022

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