Unrivalled performances 

  • Grayscale images to micropattern gradients of molecules to reproduce how neurons reach the brain
  • Subcellular precision and flexibility down to 1,2 μm
  • Long term neurons culture
  • Large surface micropatterning to promote cell guidance

Micropattern gradients of molecules to reproduce brain conditions

Grayscale micropatterning is therefore particularly suitable for quantitatively determining the chemo-attractant or chemo-repellent effect of axon guidance cues.

Chicken brain explant positioned in the center of a wheel pattern of laminin labeled with alexa488 (green). Courtesy of H. Ducuing, R. Moore, Y. Lecomte, P.-O. Strale and V. Studer.

Axon guidance on the spokes and around the circumference of the laminin wheel pattern (24-hours time-lapse video). Courtesy of H. Ducuing, R. Moore, Y. Lecomte, P.-O. Strale and V. Studer. 

Subcellular precision and flexibility 

Micropatterning allows an easier targeting of internal elements of neurons during the cryo-ET workflow. The precision and the flexibility of PRIMO offers to design multiple pattern shapes and to reproducibly position neurons on the grid. 

Primary neurons derived from the brains of 3rd instar Drosophila melanogaster larvae on patterned cryo-TEM grid. Bryan S. Sibert et al. BIORXIV, 2021.

Long term neurons culture 

It can be hard to keep neurons in culture for a long time, since they are very sensitive. You can find how to micropattern viable neurons for up to 21 days over large areas in our new application note, to study their maturation and migration.  

Epifluorescence mosaic of DIV14 hippocampal rat neurons on SynCAM1-Fc micropattern (5µm dots) with a final surface around 20 mm2 . Close up on epifluorescence images of hippocampal neurons at different days in vitro (DIV) Courtesy of Nathalie Piette at University of Bordeaux.

Neurons on a large micropatterned area 

You can create a pattern of ligands that recruit specific proteins to induce hemi-synapse formation helping to better understand the neurons subcellular organization.

Nathalie Piette - Neuron micropattern
Epifluorescence image of rat hippocampal neurons (DIV14) immunostained with Anti-SynCAM1 (Secondary antibody coupled to A488) in culture on SynCAM1-Fc micropatterns done with PRIMO (5 µm dots). Courtesy of Nathalie Piette, Cell Organ-izers Joint Lab, IINS Bordeaux and Alvéole.

The laminin grid guides the axons growth to optimize studies of neuron migration. 

Primary hippocampal neurons on a grid of laminin optimized and patterned with PRIMO. Courtesy of V. Studer and PO. Strale .

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