Micropattern gradients of molecules to reproduce brain conditions
Grayscale micropatterning is therefore particularly suitable for quantitatively determining the chemo-attractant or chemo-repellent effect of axon guidance cues.
Subcellular precision and flexibility
Micropatterning allows an easier targeting of internal elements of neurons during the cryo-ET workflow. The precision and the flexibility of PRIMO offers to design multiple pattern shapes and to reproducibly position neurons on the grid.
Long term neurons culture
It can be hard to keep neurons in culture for a long time, since they are very sensitive. You can find how to micropattern viable neurons for up to 21 days over large areas in our new application note, to study their maturation and migration.
Neurons on a large micropatterned area
You can create a pattern of ligands that recruit specific proteins to induce hemi-synapse formation helping to better understand the neurons subcellular organization.
The laminin grid guides the axons growth to optimize studies of neuron migration.
Primary hippocampal neurons on a grid of laminin optimized and patterned with PRIMO. Courtesy of V. Studer and PO. Strale .