Unrivalled performances

  • Ease experiments to study cell maturation
  • Micropattern on any substrates’ topography
  • Precise control over single cells and cell population shapes, sizes and locations
  • High content screening on adherent cells

Cell maturation

With our technology, it is possible to micropattern substrates with physiological stiffness and promote cell maturation. Protein micropatterning performed with PRIMO will help you to grow mature cells able to proliferate and differentiate into other cells.

Here, researchers manage to obtain cardiac progenitor cells capable of proliferating into cardiomyocytes from human induced pluripotent stem cells.

Fig: Cardiomyocytes derived from human induced pluripotent stem cells were cultured on laminin-coated patterns obtained with PRIMO (Alveole) to induce a specific maturation of cardiomyocytes. Nuclei (blue), sarcomeres (α-actinin, red) and gap junctions (connexin-43, green) are shown. Scale bar = 30µm Courtesy of pr. Onnik Agbulut’s lab (Sorbonne Université, Institut de Biologie Paris-Seine (IBPS), CNRS UMR 8256, Inserm ERL U116).

Micropattern on any substrates’ topography

The PRIMO technology is compatible with stiff or soft cell culture substrates with varying topographies. With our technology, cells adhere on patterned areas only, for high culture yields, in tightly controlled physical and chemical environments.

Here, the objective was to form myotubes on a culture chip connected to pillars that have a contraction monitoring capability to mimic muscular disorders. They used PRIMO to pattern around and in between duo of pillars to restrict the myoblasts adhesion in the inter-pillar area and thus avoid myoblast monolayer formation over the entire chip surface. Thus, the myoblasts adhered in clusters and fused into myotubes strictly in the axis of the pillars.

Micropatterning 3D myotubes with light induced molecular adsorption. Nicolas Rose, and al., BioRxiv 2021.
Optimization of substrate configuration for myotube culture efficiency, Nicolas Rose, and al., BioRxiv 2021.

Precise control over single cells and cell population shapes, sizes and locations

With PRIMO you can control the shape, the size and the location of your cells on the substrate to conduct homogenous and reproducible cell models.

Down below, micropatterning of adherence proteins allowed to test drugs against Rett syndrome on a homogeneous population of astrocytes. Thanks to photopatterning of large fibronectin lines, cell morphology was homogenized into polarized longitudinal cells and researchers were able to compare polymerization rate of MeCP2-deficient astrocytes with control wild type.

Human MECP2 p.Arg294* astrocytes present a higher MT growth velocity and a higher EB3-comet size than wild-type astrocytes in standardized morphology condition, that can both be corrected by EpoD, C. Delépine et al., Human Molecular Genetics, 2016.

High content screening on adherent cells

It is now possible to conduct reproducible high content screening experiments on single cells or on cell population that are isolated on your custom micropatterns. With its micrometer resolution, the PRIMO micropatterning system allows to pattern any images at a subcellular scale and cellular scale with a high reproducibility. Therefore, it permits to precisely isolate singles cells or defined areas for cell population and study them in homogeneous and standardized conditions. These high number of cells grown in the same environment enable high content screening for more accurate disease modeling or drug testing.

Left: Epifluorescence image of RPE1 cells stained for actin into 9 patterned wells. Right: Zoom on one field of view. Red: actin, Green: fibrinogen A488, Blue: nucleus.
Left: Epifluorescence image of HeLa cells stained for actin into 9 patterned wells. Right: Zoom on different patterns of cells and the corresponding projected image.

Do you have a question about your project of experiment with PRIMO?

Our research and application development team can help you set up or optimize your experimental protocols!

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