PRIMO

Custom micropatterning for
cell control

Control the chemistry and topography of cell microenvironment and study their impacts on cell development, using a contactless and maskless quantitative photopatterning solution.


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Take control of the cell microenvironment

PRIMO was developed to enable you to engineer custom in vitro microenvironments and fine-tune their mechanical and biochemical properties, at the micrometer scale with high flexibility and reproducibility.

Bioengineering cell microenvironments in vitro becomes easy.

Simply select the pattern you want to use from your computer files (no size or shape limitations). Primo then projects it onto the cell culture substrate to create microstructures and/or to generate micropatterns with the protein of your choice.

PRIMO
“The fact that we can use the same system to generate multiprotein patterns and even create 3D structures makes it a very powerful setup. “

Project Leader, Jorge Ferreira, i3S, Portugal

PRIMO micropatterning technology

Studying the influence of the microenvironment on intracellular and intercellular mechanisms has been essential for research in cell and medical biology, for many years now.

Among the methods to control this microenvironment is a rapidly developing process called “micropatterning”, which involves creating protein patterns upon which living cells are cultivated. However, current micropatterning techniques are tedious, complex and non-quantitative.

Based on this finding, the scientists at Alvéole developed an innovative multi-protein photopatterning technique to make experimental manipulations easier for researchers.

The PRIMO technique is based on LIMAP* technology (Light Induced Molecular Adsorption of Proteins) and combines a maskless and contactless photolithography system (PRIMO) controlled by a dedicated software (named “Leonardo”) and a specific photoactivatable reagent (PLPP). The combined action of UV and PLPP makes it possible to generate, in only a few seconds, any multi-protein pattern on standard cell culture substrates.

Micropatterning made easy

Discover the key steps of PRIMO photopatterning process

Pattern design

01

Pattern design

An image file is loaded in
Leonardo Software which sends it
to the PRIMO module

Illumination UV

02

UV illumination

PRIMO projects the image
onto the substrate (UV light, λ=375nm).
The pattern results from the
combined action of UV and PLPP
(photoactivatable reagent).

Micropatterning

03

Protein micropatterning

Proteins are added
(fibronectin for example) and bind to
the illuminated areas only.

Micropatterning

04

Cell adhesion

Cells are seeded
and adhere to the
protein micropatterns only.

And now go beyond micropatterning

Microfabrication with PRIMO

microfabrication

As a photopatterning system PRIMO, in addition to protein micropatterning PRIMO can also be used for microfabrication to create microstructured substrates.

From micro-pillars to wells and grooves, PRIMO allows rapid prototyping of all shapes you can imagine to create substrates adapted to each and every experiment!

 

Learn more about microfabrication with PRIMO

Unrivalled performance

Gradients

256

gray levels

Multi-protein

3

depending on
experimental conditions

High Resolution

1,2µm

over the entire field of view*

*Approximately 500×300µm,
20x objective

Fast

30sec

for a full field pattern*

*Approximately 500×300µm,
20x objective

Compatible

standard
substrates

for cell culture**

** slides, coverslips, Petri dishes,
3D devices, microfluidic devices, etc.

MULTI-PROTEIN

MULTI-PROTEIN

Sequential photopatterning of Fibrinogen-A488 in green and Protein A-A647 in red onto PDMS micro-pillars microfabricated with PRIMO.

HIGH RESOLUTION AND COMPATIBLE

HIGH RESOLUTION AND COMPATIBLE

Epifluorescence microscopy image of 1,5µm dots (spaced by 1,5µm) of ProteinA-488 on PDMS.

HIGH RESOLUTION AND COMPATIBLE

HIGH RESOLUTION AND COMPATIBLE

Epifluorescence microscopy image of 2µm horizontal lines of ProteinA-488 on glass.

GRADIENTS

GRADIENTS

Epifluorescence microscopy image of a gradient of Fibrinogen-A488 on a glass coverslip.

Benefits

Time saving 
& independence

Design and conduct
your own experiment!

High Flexibility

Draw, download and project a new image according to your needs!

Versatility

Use your regular cell culture substrates (flat or microstructured, stiff or soft) without constraints

Micropattern a wide range of molecules

More than 10 proteins used daily by our users, including
Fibrinogen-488, Fibrinogen-647, Fibronectin, GFP, Neutravidin-488, Neutravidin-647, PLL-PEG-Biotin, Protein A-647, Streptavidin, as well as primary and secondary antibodies.

Use your usual substrates

Protein patterning:
glass coverslips, glass slides, PDMS, polyacrylamide gel (transfer), polystyrene, UV-curable materials.

Microfabrication: UV-photoresist.

Applications in cell biology

“We are working on the generation of 3D cellular microenvironments to reproduce Hematopoietic Niches. PRIMO will be used to generate 3D photo-polymerized microenvironments and to pattern them to localize different cell populations involved in the hematopoiesis.”

“Our aim is to develop in vitro experimentation to decipher guiding mechanisms involved in vivo. PRIMO technology is particularly adapted to design in vitro microdevices patterned with controlled patches of the signaling proteins relevant for white blood cell migration.”

“My research focuses on the mechanical aspects of cell division. With the PRIMO system we wanted to control cellular geometry to impose spatial constraints during mitotic progression.”

“We are interested in imaging subcellular localization of certain cell-surface receptors and check whether they colocalize with focal-adhesion complexes. For this purpose, we are interested in making different types of patterns of Fibronectin with subcellular dimensions.”

“My research project aims at unravelling how a T cell switches from a fast migratory state to a stationary state upon activation. To do so, I perform live cell imaging of T cells migrating inside micro-fabricated channels coated with activating molecules. However, with this approach, I do not control when and where a T cell encounters the activating molecules.”

“Protein micropatterning represents an excellent tool to probe the behavior and functions of cellular systems. PRIMO is specially suited for our experiments, in which the cell-substrate interaction needs to be precisely adjusted both throughout the substrates and in time, in order to control the dynamic behaviour of cell monolayers.”

Resources and Support

Our team gives you all the tips to successfully conduct your experimental manipulations and go even further!

Video
tutorials

Scientific
papers

Application notes

See all resources

For an optimized and personalized control over your experimental conditions, discover complementary PRIMO products.

Request an offer
or a demo
If you would like to test PRIMO on one of your experimental projects, receive a quote or simply to know more about Alvéole and its products

Our team will be happy to answer any questions you may have.

+33 (0)1 84 25 51 41
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