LEONARDO 5

New version and new concept

The LEONARDO software has been developed to control the PRIMO system and optimize the usage of its different capabilities.

The new LEONARDO 5 is built in modules to improve its ease of use and to save time while performing experiments with PRIMO: micropatterning, EM grids micropatterning, multiwell plates micropatterning, hydrogel structuration, microfabrication, multi-images projection.

The new features and modules have been approved and validated by our users.


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LEONARDO 5 advantages

LEONARDO 5 is more user-friendly and includes many new functionalities.

It matches the user’s needs:

  • Ergonomic and handy GUI with automatized functionalities, increased
    intuitiveness & clearer icon designs
  • Step-by-step workflow to guide you in using the different modules
  • New report format allowing to save the entire information of your experiment

New functionalities have been implemented:

  • Management of microscope motorizations: in addition to the xy stage and camera, LEONARDO is now able to control the microscope objective switches. Furthermore, the z value can be saved to ensure a correct focus on the sample.
  • User session to save your own experimental parameter preferences for each module (ie doses, replications, spacing, grey level, rotation)

Teaser : how to work with LEONARDO 5

The new LEONARDO software mindset

Pick up the appropriate module for your experiments and save your experimental parameters.

EM Grids module

Hydrogel Structuration: photopolymerize UV-sensitive hydrogels by adjusting the illumination parameters (black and white, or grayscale) to obtain complex 3D structures.

 

Microfabrication: microfabricate full microchips and organ-on-a-chip by quickly loading large image files thanks to an optimized file processing.

 

Multi-image Projection: a film-like projection of patterns, where successive different images can be displayed with a chosen projection duration.

Micropatterning: the most versatile module with handy functionalities to quickly set up your photopatterning experiment on any cell culture substrate.

 

EM Grids Micropatterning: micropattern one or several EM grids in a row by saving the z value, and take advantage of the improved autodetection of the grids to improve your cryo-ET workflow.
Select a customized grid area, to position your micropatterned cells: in the center for an improved FIB milling throughput or out of damaged grid squares.

 

Multiwell Plates Micropatterning: micropattern multiwell plates by choosing a standard or customized template. Place your patterns in any well (line, column, and clubbed wells) and anywhere inside the well.

Multiwell Plates Micropatterning module

LEONARDO 5 compatibility

micromanager

1.4 version
2.0 version

OS

Windows7 to Windows11

Microscope equipment

All devices*
integrated under
µManager

*camera, stage, z motorization, objectives, fluorescence

Request an offer
or a demo
If you would like to test PRIMO on one of your experimental projects, receive a quote or simply to know more about Alvéole and its products

Our team will be happy to answer any questions you may have.

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  • This field is for validation purposes and should be left unchanged.

For an optimized and personalized control over your experimental conditions, discover all the other complementary PRIMO products.

 

PRIMO

Bioengineering unleashed

PRIMO 2, new generation with compact footprint, new optical design allowing epi-fluorescence microscopy and faster performances. Micropatterning, hydrogel polymerization and microfabrication, all in a single device. Create bespoke in vitro cellular microenvironments and get better cell models for your cell biology experiments or cryo-ET studies.
Discover Primo
 

PDMS Stencil

The multi-well solution

With its multiple wells, the PDMS Stencil reduces the volumes of reagents used and accommodates different experimental conditions on the same substrate.
Discover the PDMS Stencil
 

PRIMO REAGENTS

The photoactivatable product line

PRIMO is a UV-based photopatterning system with a diverse range of applications including micropatterning and hydrogel structuration. Our product line supports both of these applications with photoactivable reagents. PLPP Classic: enables high-throughput micropatterning of cell culture substrates when illuminated with PRIMO. PLPP Gel: accelerates the speed of your micropatterning experiments and substrate biofunctionalization up to 30 times compared to PLPP Classic. HyPE: enables to flexibly structure hydrogels in 2.5D, while coupled with UV.
Additional information on the PRIMO reagents
 

PRIMO OPTICAL PLATFORM

We are now offering a PRIMO PLATFORM with already integrated optics. This platform gives you access to the PRIMO2 technology without the need for a dedicated microscope. An all-in-one platform with two different configurations available: - the MICROPATTERNING PRIMO PLATFORM dedicated to micropatterning - the ADVANCED PRIMO PLATFORM for all applications of micropatterning, hydrogel polymerization, and microfabrication.  
Additional information on the PRIMO reagents

Resources and Support

Our team gives you all the tips to successfully conduct your experimental manipulations and go even further!

Video
tutorials

Scientific
papers

Application notes

See all resources