LEONARDO 5

New version and new concept

The LEONARDO software has been developed to control the PRIMO system and optimize the usage of its different capabilities.

The new LEONARDO 5 is built in modules to improve its ease of use and to save time while performing experiments with PRIMO: micropatterning, EM grids micropatterning, multiwell plates micropatterning, hydrogel structuration, microfabrication, multi-images projection.

The new features and modules have been approved and validated by our users.

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LEONARDO 5 advantages

LEONARDO 5 is more user-friendly and includes many new functionalities.

It matches the user’s needs:

Ergonomic and handy GUI with automatized functionalities, increased
intuitiveness & clearer icon designs
Step-by-step workflow to guide you in using the different modules
New report format allowing to save the entire information of your experiment

New functionalities have been implemented:

Management of microscope motorizations: in addition to the xy stage and camera, LEONARDO is now able to control the microscope objective switches. Furthermore, the z value can be saved to ensure a correct focus on the sample.
User session to save your own experimental parameter preferences for each module (ie doses, replications, spacing, grey level, rotation)

Teaser : how to work with LEONARDO 5

The new LEONARDO software mindset

Pick up the appropriate module for your experiments and save your experimental parameters.

EM Grids module

Hydrogel Structuration: photopolymerize UV-sensitive hydrogels by adjusting the illumination parameters (black and white, or grayscale) to obtain complex 3D structures.

Microfabrication: microfabricate full microchips and organ-on-a-chip by quickly loading large image files thanks to an optimized file processing.

Multi-image Projection: a film-like projection of patterns, where successive different images can be displayed with a chosen projection duration.

Micropatterning: the most versatile module with handy functionalities to quickly set up your photopatterning experiment on any cell culture substrate.

EM Grids Micropatterning: micropattern one or several EM grids in a row by saving the z value, and take advantage of the improved autodetection of the grids to improve your cryo-ET workflow.
Select a customized grid area, to position your micropatterned cells: in the center for an improved FIB milling throughput or out of damaged grid squares.

Multiwell Plates Micropatterning: micropattern multiwell plates by choosing a standard or customized template. Place your patterns in any well (line, column, and clubbed wells) and anywhere inside the well.