Fields of application

Adjust the biochemical and mechanical cues of the cell microenvironment in vitro with PRIMO and generate more physiologically-relevant cell models.

The system allows you to better study the behavior and development of living cells in a broad range of applications, such as: cytoskeleton dynamics, cell adhesion force measurement, cell confinement, cell migration, tissue engineering, spheroids.

Micropatterning

Micropatterning

Workflow principle

Intra-cellular mechanisms

Tune pattern geometries to impose different cell shapes to study intracellular cytoskeleton organization

Neurons and Guidance

Conduct axon guidance experiments and study neurons in conditions as close as possible to their in vivo state.

Easily induce and study cell migration by quickly creating any adhesive shape. Standardize studies on collective or individual cell migration.

Disease modeling

Tune complex microenvironment with 2.5D topographies and diverse cell populations in order to obtain reproducible disease models.

Cell force measurements

Create more reproducible models to mimic physiological or pathological cell forces.

Cell behavior on topological cues

Control micropatterning alignment on existing topographies of cell culture substrates to study cell behavior on topological environments.

Cell positioning for Cryo-ET

Create perfect sample for FIB-milling and electron Cryo-tomography by directly controlling cell adhesion, localization and shape.

Microfabrication

Microfabrication

Workflow principle

Organ-on-a-chip production

Prototype your whole organ-on-a-chip in a record time and with a high reproducibility.

Grayscale photolithography

Microfabricate complex structures like ramps and stairs with projection of gray levels.

Microstructured substrate

Create your microstructured substrates with all the geometries and topographies you can imagine, including micropillars, microwells or grooves.

Combining approaches

Control the cell microenvironment from the biophysical properties of the substrate to the biochemical parameters to create complex and relevant models.

Hydrogel structuration

Hydrogel structuration

Workflow principle

3D Cell Culture

Polymerize photosensitive hydrogels to create structured substrates for your 3D cell culture models.

Spheroids and organoids microwells

Grow reproducible 3D cell aggregates, control their shape, and organize them in space for a precise automated data analysis.

Microfluidic chips in situ photopolymerization

Structure UV sensitive hydrogels inside microchannels.

Functionalization of hydrogels

Combine protein micropatterning and hydrogel polymerization to turn common hydrogels into topographically and chemically heterogeneous environments.