PDMS Stencil

The multi-well solution

With its multiple wells, the PDMS Stencil reduces the volumes of reagents used and accommodates different experimental conditions on the same substrate.

Several wells, several possibilities

Because research work can only be successful after many tests and continuous optimization, we have developed a multi-well membrane, so you can multiply your experimental conditions on the same substrate and save on reagents.

The PDMS Stencil is thin and flexible, making it easy to use and place on all flat substrates (glass, plastic, or PDMS).

Several uses and formats are available:
  • Multi-wells
  • Hydrogel polymerization
  • Flow chamber
  • EM grid handling

For very specific requirements, we can also design tailor-made models.

2d-Stencil

4 wells

picto PDMS Stencil 4 wells

Ø ext 15 mm,
Ø puits : 4 mm

9 wells

picto PDMS Stencil 9 wells

ext: 20×20 mm,
puits : 3×3 mm

Hydrogels

picto PDMS Stencil Hydrogels

Ø ext 18.5mm

Flow Chamber

picto PDMS Stencil Flow chamber

18×18 mm

EM grid

picto PDMS Stencil EM grid

3 mm

How to use the PDMS Stencil

1. Lift the plastic protective film to reveal one of the PDMS Stencils. Remove the PDMS from the well using needle-nose pliers.

2d-PDMS-stencil-step1

2. Pinch one of the PDMS Stencil corners and remove it from the sheet. Clean it with 70% ethanol and dry it (with warm blown air for example).

2d-PDMS-stencil

3. Place the PDMS Stencil on a clean substrate (after plasma treatment if required) and remove any air bubbles by tapping it.

2d-PDMS-stencils

4. Add your reagents in the various wells and carry out your micropatterning experiments.

PDMS-stencil

Multiple uses, just like your experimental tests

Confines

liquids*

*doesn’t leak on
the microscope lens

Saves

volume of reagent

used per experiment

Multiplies

experimental conditions

on a substrate

Localizes

experimental zones

protein and cellular micropatterns

Resources and Support

Our team gives you all the tips to successfully conduct your experimental manipulations and go even further!

Video
tutorials

Scientific
papers

Application notes

See all resources

For an optimized and personalized control over your experimental conditions, discover complementary PRIMO products.

 

PRIMO

Bioengineering unleashed

PRIMO 2, new generation with compact footprint, new optical design allowing epi-fluorescence microscopy and faster performances. Micropatterning, hydrogel polymerization and microfabrication, all in a single device. Create bespoke in vitro cellular microenvironments and get better cell models for your cell biology experiments or cryo-ET studies.
Discover Primo
 

PRIMO REAGENTS

The photoactivatable product line

PRIMO is a UV-based photopatterning system with a diverse range of applications including micropatterning and hydrogel structuration. Our product line supports both of these applications with photoactivable reagents. PLPP Classic: enables high-throughput micropatterning of cell culture substrates when illuminated with PRIMO. PLPP Gel: accelerates the speed of your micropatterning experiments and substrate biofunctionalization up to 30 times compared to PLPP Classic. HyPE: enables to flexibly structure hydrogels in 2.5D, while coupled with UV.
Additional information on the PRIMO reagents
 

LEONARDO 5

New version and new concept

The LEONARDO software has been developed to control the PRIMO system and optimize the usage of its different capabilities. The new LEONARDO 5 is built in modules to improve its ease of use and to save time while performing experiments with PRIMO: micropatterning, EM grids micropatterning, multiwell plates micropatterning, hydrogel structuration, microfabrication, multi-images projection. The new features and modules have been approved and validated by our users.
Discover the features of LEONARDO
 

PRIMO OPTICAL PLATFORM

We are now offering a PRIMO PLATFORM with already integrated optics. This platform gives you access to the PRIMO2 technology without the need for a dedicated microscope. An all-in-one platform with two different configurations available: - the MICROPATTERNING PRIMO PLATFORM dedicated to micropatterning - the ADVANCED PRIMO PLATFORM for all applications of micropatterning, hydrogel polymerization, and microfabrication.  
Additional information on the PRIMO reagents
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