Alvéole - Take care of your cells

Take care of your cells

Primo

The first multi-protein photopatterning solution

About

Key applications for major challenges, Alvéole awarded a prize at WIC 2014

News

09.2016

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Primo

The first multi-protein photopatterning solution

Primo is an innovative solution for patterning proteins on cell culture dishes. Directly mounted on an inverted microscope, the device consists of an optical illumination module coupled with the use of PLPP, a photo-activator molecule. The image to be patterned is projected by a UV laser through the microscope in the presence of PLPP, which catalyses the UV effect. The protein is then added and will bind to the illuminated areas.

Device

Directly connected to a microscope

Technology

Principle of protein photopatterning

Applications

Primo was developed to enable you to design and conduct all the micropatterning experiments you can imagine, in 2D and also 3D. Simply select from amongst your computer’s files the pattern you want to use (no size or shape limitations). Primo then projects it on the cell culture substrate and allows you to generate the pattern with the protein of your choice.

We are gradually discovering the extent of the fields of application of this new technology and we are pleased to show you a few examples of some early experiments conducted by our users.

Custom micropatterning

Mouse teratocarcinoma cells plated on fibronectin micropatterns.

Single cell micropatterning

Fibroblasts plated on fibronectin micropatterns (after 2 weeks).

Cell co-culture

S180 cells and MEF cells successively seeded on a Yin & Yang pattern (Yin : fibronectin, Yang : streptavidin incubated with biotinylated fibronectin).(1)

Dynamics of cellular mechanotransduction

On a Yin (fibronectin) and Yang (streptavidin) pattern, a single MEF Cell only adheres on fibronectin. Then biotynilated fibronectin is added and the MEF cell starts adhering on the entire pattern.(1)

Patterning and neurobiology

Astrocyte-based assays, 1st without patterning
Astrocytes plated on a uniform fibronectin coating show heterogeneity in shapes.(2)
The microtubules were tagged with GFP, but their growth direction was heterogeneous and couldn’t be measured.(2)
Astrocyte-based assays, with patterning
Astrocytes were plated on fibronectin lines.(2)
The microtubules were tagged with GFP and then shown the same orientation, which allowed to measure microtubule growth rates.(2)
Axon guidance
Chicken brain explant positioned in the center of a wheel pattern of laminin labelled with alexa488 (green).(3)
Axon guidance on the spokes and around the circumference of the laminin wheel pattern (24 hours time-lapse video).(3)

Micro-organ-on-a-chip

3 hepatocytes HepG2 adhering on patterns of fibronectin on the sides and the bottom of a micro-well.(4)
  1. Courtesy of V. Studer and P.-O. Strale.
  2. Courtesy of C. Delépine. ”Altered microtubule dynamics and vesicular transport in mouse and human MeCP2-deficient astrocytes“, Delépine C. et al., Hum. Mol. Genet. 2015.
  3. Courtesy of H. Ducuing, R. Moore, Y. Lecomte, P.-O. Strale and V. Studer.
  4. Courtesy of C. Stoecklin and V. Viasnoff

Specifications

Primo brings you unrivalled performance

  • Multiple proteins photopatterning (up to 3 without restrictions)
  • Quantitative photopatterning (256 levels of grey)
  • Resolution of 1.2 µm over the entire field of view
  • Fast (20s for a full field pattern)
  • Compatible with standard cell culture substrates

Primo software key features

  • Automatic calibration
  • Pattern replication
  • Patterning of large images
  • Realignment of successive patterns or on 3D structures

About

Alvéole: key applications for major challenges

The rapid evolution of cell biology discoveries leads to a growing need among scientists for innovative tools. Founded in December 2010, Alvéole offers solutions that combine the latest innovations in cell imaging, microfluidics and nanotechnology to enhance control the development and proliferation of living culture cells.

The future

Alvéole’s devices control various parameters of the cellular microenvironment and assess their influence on cell development. These devices are particularly suitable for stem cell experiments, cell-based assays for drug discovery and predictive toxicology to reduce animal testing and address major public health issues:

Cancer
Diabetes
Immunology
Neurobiology
Regenerative Medicine

Prize-winning project

Alvéole was a prize winner at the Worldwide Innovation Challenge for its CellForLife project in the individualised medicine category. This project aims to develop a complete offering with a handling platform and a miniaturised device to control stem cell differentiation, in order to develop personalised treatments for patients suffering from genetic diseases. As part of their research into Rett syndrome, researchers at the Cochin Institute use the Alvéole tools to understand the disease and develop specific therapies.

SLAS : Primo wins the 2016 New Product Award

SLAS (Society for Laboratory Automation and Screening) is a global community of more than 18,000 scientists—from academia, government and industry—collectively focused on leveraging the power of technology to achieve scientific objectives. 320 exhibitors participated in the 2016 SLAS conference in San Diego. During the event, the new products have been assessed for their innovative nature and their impact in the field of laboratory techniques. Primo wins the 2016 New Product Award.

Team

Born from the meeting between two biophysicists, Maxime Dahan, a cell imaging specialist, and Vincent Studer, a microfluidic specialist, Alvéole is a subsidiary of Quattrocento, the creator of innovative technology companies in the field of life sciences.

Luc Talini - CEO, Aurélien Duboin - Application Engineer, Josselin Ruaudel - Product Development, Matthieu Opitz - Cell Biology, Louise Bonnemay - Application Development, Marie-Charlotte Manus - Communication Manager, David Poizat - Technical Director of Quattrocento, Bertrand Fourquet - President of Quattrocento.

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