Alvéole - Take care of your cells

Take care of your cells

Primo

The first multi-protein printing platform

About

Key applications for major challenges, Alvéole awarded a prize at WIC 2014

News

06.2016

Alvéole will be attending the Physiology summer school of the Marine Biological Laboratory in Woods Hole from June 20 to July 30, 2016 where Primo its protein photo-patterning technology will be tested

Read all news…

Primo

The first multi-protein printing platform

Primo is an innovative solution for printing proteins on cell culture dishes. Directly mounted on an inverted microscope, the device consists of an optical illumination module coupled with the use of PLPP, a photo-activator molecule. The image to be printed is projected by a UV laser through the microscope in the presence of PLPP, which catalyses the UV effect. The protein is then added and will bind to the illuminated areas.

Device

Directly connected to a microscope

Technology

Principle of protein printing

Achievements

Protein printing

Projected image
Image appears in real time when GFP is deposited on the printed pattern (real size: 50µm)
Projected image
Image obtained by printing three fluorescent proteins (PEG-FITC, PEG-TRITC, PEG-Ato 647) successively deposited

Applications

Tissue engineering

Rectangular patterns of Fibronectin
Fibroblasts culture on the patterns of the previous figure
Addition of a gradient area
The arrangement of the cell culture is influenced by the gradients
Pattern
A polylysine grid combined with slits guide the development of neurons in culture.

Specifications

Primo brings you unrivalled performance

  • Compatible with high-resolution microscopy
  • Multiple printing (up to 3 without restrictions)
  • Resolution of 1.2 µm over the entire field of view
  • Quantitative printing
  • Fast printing (20s for a full field pattern)

Primo software key features

  • Automatic calibration
  • Multi-patterning
  • Management of large images
  • Alignment of successive patterns

About

Alvéole: key applications for major challenges

The rapid evolution of cell biology discoveries leads to a growing need among scientists for innovative tools. Founded in December 2010, Alvéole offers solutions that combine the latest innovations in cell imaging, microfluidics and nanotechnology to enhance control the development and proliferation of living culture cells.

The future

Alvéole’s devices control various parameters of the cellular microenvironment and assess their influence on cell development. These devices are particularly suitable for stem cell experiments, cell-based assays for drug discovery and predictive toxicology to reduce animal testing and address major public health issues:

Cancer
Diabetes
Immunology
Neurobiology
Regenerative Medicine

Prize-winning project

Alvéole was a prize winner at the Worldwide Innovation Challenge for its CellForLife project in the individualised medicine category. This project aims to develop a complete offering with a handling platform and a miniaturised device to control stem cell differentiation, in order to develop personalised treatments for patients suffering from genetic diseases. As part of their research into Rett syndrome, researchers at the Cochin Institute use the Alvéole tools to understand the disease and develop specific therapies.

SLAS : Primo wins the 2016 New Product Award

SLAS (Society for Laboratory Automation ans Screening) is a global community of more than 18,000 scientists—from academia, government and industry—collectively focused on leveraging the power of technology to achieve scientific objectives. 320 exhibitors participated in the 2016 SLAS conference in San Diego. During the event, the new products have been assessed for their innovative nature and their impact in the field of laboratory techniques. Primo wins the 2016 New Product Award.

Team

Born from the meeting between two biophysicists, Maxime Dahan, a cell imaging specialist, and Vincent Studer, a microfluidic specialist, Alvéole is a subsidiary of Quattrocento, the creator of innovative technology companies in the field of life sciences.

Luc Talini - CEO, Aurélien Duboin - Application Engineer, Josselin Ruaudel - Product Development, Matthieu Opitz - Cell Biology, Louise Bonnemay - Application Development, Marie-Charlotte Manus - Web Communication Manager, David Poizat - Technical Director of Quattrocento, Bertrand Fourquet - President of Quattrocento.

Partners