Alvéole - Take care of your cells

Take care of your cells

Primo

The first multi-protein printing platform

R&D

Tools to control the microenvironment at the level of a single cell

About

Key applications for major challenges, Alvéole awarded a prize at WIC 2014

News

05.2016

Alvéole will be attending the Advances in Cell & Tissue Culture 2016 Meeting in Barcelona from May 30 to June 1st, 2016 to present Primo, its protein photo-patterning technology

Primo

The first multi-protein printing platform

Primo is an innovative solution for printing proteins on cell culture dishes. Directly mounted on an inverted microscope, the device consists of an optical illumination module coupled with the use of PLPP, a photo-activator molecule. The image to be printed is projected by a UV laser through the microscope in the presence of PLPP, which catalyses the UV effect. The protein is then added and will bind to the illuminated areas.

Device

Directly connected to a microscope

Technology

Principle of protein printing

Achievements

Protein printing

Projected image
Image appears in real time when GFP is deposited on the printed pattern (real size: 50µm)
Projected image
Image obtained by printing three fluorescent proteins (PEG-FITC, PEG-TRITC, PEG-Ato 647) successively deposited

Applications

Tissue engineering

Rectangular patterns of Fibronectin
Fibroblasts culture on the patterns of the previous figure
Addition of a gradient area
The arrangement of the cell culture is influenced by the gradients
Pattern
A polylysine grid combined with slits guide the development of neurons in culture.

Specifications

Primo brings you unrivalled performance

  • Compatible with high- and super-resolution microscopy
  • Multiple printing (up to 3 without restrictions)
  • Resolution <1 µm over the entire field of view
  • Quantitative printing (255 levels of grey)
  • Fast printing (20s for a full field pattern)

Primo software key features

  • Automatic calibration
  • Multi-patterning
  • Management of large images
  • Alignment of successive patterns

R&D

Tools to control the microenvironment at the level of a single cell

Based on several innovations in microfluidics, nanotechnology and super-resolution microscopy, Alvéole is developing a portfolio of miniaturised devices for controlling various cellular micro-environment parameters. These devices offer many advantages:

For each application, Alvéole offers solutions that can be combined for even more advanced control of the cellular microenvironment.

Micrograd
The Controlled gradients generator

Micrograd is a miniaturised device for generating a steady gradient of concentration above the cell cultures. The device was used and validated in neuronal navigation experiments (cf. references).

Specifications:

+-References

MarChip
Control and drive stem cell differentiation

The MARChips are miniaturised devices consisting of a matrix of 36 patterns each with a different topology (grooves, pads, holes etc.). Depending on the pattern, the cultured cells will not behave in the same way, in particular the stem cells that will differentiate into different types according to the pattern. The device has been validated in differentiation tests from stem cells into neural cells (cf. references).

Specifications:

+-References

About

Alvéole: key applications for major challenges

The rapid evolution of cell biology discoveries leads to a growing need among scientists for innovative tools. Founded in December 2010, Alvéole offers solutions that combine the latest innovations in cell imaging, microfluidics and nanotechnology to enhance control the development and proliferation of living culture cells.

The future

Alvéole’s devices control various parameters of the cellular microenvironment and assess their influence on cell development. These devices are particularly suitable for stem cell experiments, cell-based assays for drug discovery and predictive toxicology to reduce animal testing and address major public health issues:

Cancer
Diabetes
Immunology
Neurobiology
Regenerative Medicine

Prize-winning project

Alvéole was a prize winner at the Worldwide Innovation Challenge for its CellForLife project in the individualised medicine category. This project aims to develop a complete offering with a handling platform and a miniaturised device to control stem cell differentiation, in order to develop personalised treatments for patients suffering from genetic diseases. As part of their research into Rett syndrome, researchers at the Cochin Institute use the Alvéole tools to understand the disease and develop specific therapies.

SLAS : Primo wins the 2016 New Product Award

SLAS (Society for Laboratory Automation ans Screening) is a global community of more than 18,000 scientists—from academia, government and industry—collectively focused on leveraging the power of technology to achieve scientific objectives. 320 exhibitors participated in the 2016 SLAS conference in San Diego. During the event, the new products have been assessed for their innovative nature and their impact in the field of laboratory techniques. Primo wins the 2016 New Product Award.

Team

Born from the meeting between two biophysicists, Maxime Dahan, a cell imaging specialist, and Vincent Studer, a microfluidic specialist, Alvéole is a subsidiary of Quattrocento, the creator of innovative technology companies in the field of life sciences.

Luc Talini - CEO, Aurélien Duboin - Application Engineer, Josselin Ruaudel - Product Development, Matthieu Opitz - Cell Biology, Louise Bonnemay - Application Development, Marie-Charlotte Manus - Web Communication Manager, David Poizat - Technical Director of Quattrocento, Bertrand Fourquet - President of Quattrocento.

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