Alvéole - Take care of your cells

Take care of your cells

Primo

The first multi-protein photopatterning solution

About

Key applications for major challenges, Alvéole awarded a prize at WIC 2014

News

07.2016

Are you attending the International Congress of Cell Biology, in Prague from July 20 to July 25, 2016 ? Come and meet us on BOOTH 15 where we present PRIMO and its applications!

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Primo

The first multi-protein photopatterning solution

Primo is an innovative solution for patterning proteins on cell culture dishes. Directly mounted on an inverted microscope, the device consists of an optical illumination module coupled with the use of PLPP, a photo-activator molecule. The image to be patterned is projected by a UV laser through the microscope in the presence of PLPP, which catalyses the UV effect. The protein is then added and will bind to the illuminated areas.

Device

Directly connected to a microscope

Technology

Principle of protein photopatterning

Achievements

Protein photopatterning

Projected image
Image appears in real time when GFP is deposited on the projected pattern (real size: 50µm)
Projected image
Image obtained by photopatterning three fluorescent dyes (PEG-FITC, PEG-TRITC, PEG-Ato 647) successively deposited

Applications

Tissue engineering

Rectangular patterns of Fibronectin
Fibroblasts culture on the patterns of the previous figure
Addition of a gradient area
The arrangement of the cell culture is influenced by the gradients
Pattern
A polylysine grid combined with slits guide the development of neurons in culture.

Specifications

Primo brings you unrivalled performance

  • Compatible with high-resolution microscopy
  • Compatible with standard cell culture substrates
  • Multiple proteins photopatterning (up to 3 without restrictions)
  • Resolution of 1.2 µm over the entire field of view
  • Quantitative photopatterning (256 levels of grey)
  • Fast (20s for a full field pattern)

Primo software key features

  • Automatic calibration
  • Pattern replication
  • Patterning of large images
  • Realignment of successive patterns

About

Alvéole: key applications for major challenges

The rapid evolution of cell biology discoveries leads to a growing need among scientists for innovative tools. Founded in December 2010, Alvéole offers solutions that combine the latest innovations in cell imaging, microfluidics and nanotechnology to enhance control the development and proliferation of living culture cells.

The future

Alvéole’s devices control various parameters of the cellular microenvironment and assess their influence on cell development. These devices are particularly suitable for stem cell experiments, cell-based assays for drug discovery and predictive toxicology to reduce animal testing and address major public health issues:

Cancer
Diabetes
Immunology
Neurobiology
Regenerative Medicine

Prize-winning project

Alvéole was a prize winner at the Worldwide Innovation Challenge for its CellForLife project in the individualised medicine category. This project aims to develop a complete offering with a handling platform and a miniaturised device to control stem cell differentiation, in order to develop personalised treatments for patients suffering from genetic diseases. As part of their research into Rett syndrome, researchers at the Cochin Institute use the Alvéole tools to understand the disease and develop specific therapies.

SLAS : Primo wins the 2016 New Product Award

SLAS (Society for Laboratory Automation and Screening) is a global community of more than 18,000 scientists—from academia, government and industry—collectively focused on leveraging the power of technology to achieve scientific objectives. 320 exhibitors participated in the 2016 SLAS conference in San Diego. During the event, the new products have been assessed for their innovative nature and their impact in the field of laboratory techniques. Primo wins the 2016 New Product Award.

Team

Born from the meeting between two biophysicists, Maxime Dahan, a cell imaging specialist, and Vincent Studer, a microfluidic specialist, Alvéole is a subsidiary of Quattrocento, the creator of innovative technology companies in the field of life sciences.

Luc Talini - CEO, Aurélien Duboin - Application Engineer, Josselin Ruaudel - Product Development, Matthieu Opitz - Cell Biology, Louise Bonnemay - Application Development, Marie-Charlotte Manus - Web Communication Manager, David Poizat - Technical Director of Quattrocento, Bertrand Fourquet - President of Quattrocento.

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