Booth 319
Visit our Booth and ask all the questions you have about PRIMO and its applications to our team!
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Exhibitor presentation

Sunday, February 18: 12:30pm – 2:00pm / Room 6

Maskless Quantitative Multi-protein Photopatterning to orchestrate cellular microenvironment

Pierre-Olivier Strale, PhD – Senior Scientist, Alvéole (France)

Abstract here

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With invited research presentations
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Micropatterning promotes coordinated cardiomyocyte contraction

Niccole Schaible, PhD – Postdoctoral Fellow, Beth Israel Deaconess Medical Center (USA)

Abstract: “Contractile force generation is central to the physiological function of the heart. Using a high-throughput, high-content technique for measuring contractile force, we are able to investigate the collective beating of a cardiomyocyte monolayer derived from human stem cells. Although these cells form a functional syncytium, which collectively contractions and relaxes in sync, the transmission of force between neighboring cells lacks the spatial organization characteristic of in-vivo cardiomyocytes. We hypothesize that guiding these cardiomyocytes to assume a more uniform orientation, using a novel micropatterning approach (PRIMO, Alvéole, France), will not only improve the sensitivity of our contractile force screen but also enable in-depth studies regarding the biophysical mechanisms underlying transmission of force in a cardiomyocyte monolayer.​”

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Manipulating the microphysiological environment to study mechanobiology at the single cell level

Gaspard Pardon, PhD – Postdoctoral Fellow Department of Bioengineering & Mechanical Engineering & Cardiovascular Institute, Stanford University, CA. (USA)

Abstract: “Fine control and manipulation of the microphysiological environment is critical to study the many intricate mechanisms involved in mechanosignaling. Further, the ability to work at the single cell level allows for dissecting the variability found in many cell populations and to probe the mechanosensing pathways with more specificity. Here, we apply the Alveole PRIMO capabilities to the creation of multiprotein patterns to engineer the shape of single cardiomyocytes (CM) derived from human-induced pluripotent stem cells (hiPSC). These patterns are transferred into hydrogel with controlled mechanical properties to mimic the human (patho-)physiological environment.  We show how we further apply the instrument capabilities for patterning inside multiwell plates format, onto 3D-microstructures and onto electron microscopy grids.”
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Poster

Meet us at the poster session “Bioengineering I”
Poster B651: Maskless Quantitative Multi-protein Photopatterning to orchestrate cellular microenvironment
Sunday, February 18: from 1:45 pm to 2:45 pm

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