The main interest of the Tardieux’s laboratory and my PhD project is to decipher how forces drive the unique motile and invasive capacities of the single-celled eukaryotic parasite Toxoplasma gondii. I was able to uncover that the parasite glides by coupling polar adhesions and de-adhesion with traction and dragging forces. The PRIMO technique was needed to create composite patterns with a non-adhesive area next to an adhesive one with the crucial request of a sharp demarcation.