Key steps of micropatterning on microstructures with PRIMO

Whether on commercially available three-dimensional substrates or those you manufactured yourself, PRIMO allows you to micropattern your biomolecules for creating more complex in vitro cell microenvironment and better 3D cell culture systems!

For an easier control over your experiments, Leonardo software automatically detects microstructures to precisely align and project your images onto it.

Combining structuration and functionalization for more relevant in vitro cell microenvironments

First, start by generating your substrate with the microstructures and/or the stiffness you want, using PRIMO for microfabrication and/or hydrogel structuration.

Then, functionalize the surface of that substrate with your biomolecule by micropatterning it on the areas you specifically define.

Finally, you have it: a complex and more relevant in vitro cell microenvironment!

Left panel: schematic representation of 4-arm-PEG-hydrogels (blue) functionalized with Poly-L-lysin(red) and fibronectin. Middle panel: Z-color-coded image of COS-7 cells seeded on the gel and stained for actin. Right panel: 3-color z-stack showing the patterned molecules (red), the actin cytoskeleton (green) and the nuclear envelope (blue). A. Pasturel et al., BioRxiv, 2018

Top and bottom of PDMS micro-pillars (100 μm high, spaced by 100 μm) patterned with two different biomolecules.

sequential-photopatterning
Sequential photopatterning of Fibrinogen-A488 in green and Protein A-A647 in red onto PDMS micro-pillars microfabricated with PRIMO.

Do you have a question about your project of experiment with PRIMO?

Our research and application development team can help you set up or optimize your experimental protocols!

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