Key steps of the microfabrication process

using PRIMO and Leonardo software to create microstructured substrates

photopatterning-system

Microfabrication of classic microstructures:  micro-pillars, wells or grooves

The PRIMO system can microfabricate all the shapes you imagine down to a resolution of 2 µm.

photolithography-microfabrication
Micro-pillars of 5 µm diameter spaced by 5 µm made by the replica molding of a primary SU8 photoresist master obtained by photolithography with PRIMO.
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Sequential photopatterning of Fibrinogen-A488 in green and Protein A-A647 in red onto PDMS micro-pillars microfabricated with PRIMO.

And go even further with the microfabrication of complex structures in 3D!

The projection of gray levels by the PRIMO system allows for the microfabrication of complex structures such as stairs, linear or curved ramps, etc.

Pattern loaded into Leonardo software to do microfabrication in gray levels.
Optical profilometer analysis of the positive resin after illumination in gray levels by PRIMO and after development.

Experiment conducted with Institut Pierre-Gilles de Gennes.

Hydrogel photopolymerization with PRIMO

PEG-biotin successively photopolymerized with PRIMO
PEG-DA mixed with PEG-FITC (green), PEG-TRITC (blue) or PEG-biotin successively photopolymerized with PRIMO. Streptavidin incubated afterward specifically stains the outer surface of the PEG-biotin hydrogel (red). Courtesy of C. Stoecklin and V. Viasnoff
"Tailoring 3D cell culture templates with common hydrogels", A. Pasturel et al., BioRxiv, 2018 "Left panel: Schematic representation of a hydrogel flow spiral with glass slide (gray) 4-arm-PEG-acryl hydrogel (blue) and PDMS ceiling (red). Middle panel: bright field microscopy image of the resulting hydrogel. Right Panel: long exposure (1sec) epifluorescence microscopy image of the same channel incubated with flowing fluorescent beads."

Hydrogel photo-scission with PRIMO

"Tailoring 3D cell culture templates with common hydrogels", A. Pasturel et al., BioRxiv, 2018 "Left panel: Schematic representation of the photo-scission of a photopolymerized 4-arm-PEG-acrylate (blue) hydrogel. Middle Panel: bright field microscopy image of the hydrogel before and after photo-scission. Right Panel: 3D reconstruction of the final structure."

Do you have a question about your project of experiment with PRIMO?

Our research and application development team can help you set up or optimize your experimental protocols!

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