We’ve been challenged a lot for PRIMO to be used for high content screening experiments and cell-based assays. A major drawback until recently was the long illumination time that prevented from using PRIMO for high-throughput micropatterning …
Micropatterning of protein is a powerful technology to control the spatial organization of cells and their biochemical microenvironment. We have developed PRIMO: a contactless and maskless UV projection system to facilitate micropatterning experiments. However, a major drawback until recently was the long illumination time that prevented from using PRIMO for high content screening experiments and cell-based assays. Here we present a new photo-initiator (PLPP Gel) and protocol allowing fast micropatterning of protein inside standard 96 well plates.
We are proud to collaborate with Leica Microsystems to ensure you obtain the best cell samples for cryo-ET! PRIMO micropatterning + Leica cryo-CLEM = Advanced workflow for whole cell cryo-ET
Learn more on this partnership.
Ask your questions live to Dr Leeya Engel during our sponsored Nature Research webcast on « Micropatterning on EM grids: A strategy for improving cell cryo-ET workflow » > Controlling cell positioning within EM grids. Attend our webinar.
Here comes another paper using PRIMO contactless micropatterning on TEM Grids! « Tailoring cryo-electron microscopy grids by photo-micropatterning for in-cell structural studies », M. Toro-Nahuelpan et al, BioRxiv, 2019. From Julia Mahamid’s team at EMBL.
Read the paper.
« Substrate area confinement is a key determinant of cell velocity in collective migration », Mohammed et al, Nature Physics, 2019. –> They used micropatterning to mimic the spatial confinement experienced by follower cells within epithelial tissues.
Access the research paper.
Aurélien Pasturel (PhD candidate, CNRS, Alvéole) presents us his work: « Tailoring 3D cell culture templates with common hydrogels », A Pasturel, BioRxiv, 2018
Watch the scientific video.
What’s the common point between a laboratory mouse and a Petri dish? See the answer in video.
Giant cells moving on a building and adapting to obstacles. We are amazed by the CytoMorphoLab team’s creativity and how they used PRIMO bioengineering technology to miniaturise a building to the size of cells!
Find more details about the whole project on their website & make sure you watch THE VIDEO!
Are you interested in understanding the role of the extracellular matrix in cell morphology? This application note is for you.
Check how we worked with Nanosurface Biomedical to micropattern their microstructured substrate.
Using quantitative antibodies micropatterning with PRIMO and RICM imaging, the team studied haptotaxis of T lymphocytes.
See all our users’ scientific papers.
Did you miss our live webinar presented by Dr. Manuel Théry and Dr. Harikrishnan Parameswaran? They explained how PRIMO allows to work with controlled and reproducible microenvironments in vitro to more efficiently study living cells and model diseases.
Register and watch the replay on Nature dedicated webpage.
Last week we were at The University of Geneva to instal PRIMO after a successful demo!
Pau Guillamat from Aurélien Roux’ lab uses PRIMO contactess and maskless micropatterning to study epithelial dynamics.
Check some of his previous work with PRIMO.
The team who loaned a PRIMO for this year Physiology Summer Course at the Marine Biological Laboratory made quite an experiment!
To study cell junctions under curvature, they patterned MDCK cells on a spiral fibronectin pattern.
Check the video.
« Controlling the topography and biochemistry of cell culture substrates with PRIMO »
One of the challenges confronting cell biologists is to mimic the micro-environment in vitro to more efficiently study living cells. (…) PRIMO allows to tailor cell microenvironment topography via microfabrication and biochemistry via micropatterning…
Read the application note
When you offer cells a choice… Yes they go for the higher protein density! Experiment conducted by Pau Guillamat from Aurelien Roux’ lab – University of Geneva.
Watch the video of cells migrating towards two different protein densities in Pau Guillamat’s testimonial.
Virgile Viasnoff‘s lab at the Mechanobiology Institute – National University of Singapore is going further in controlling cell microenvironment in 3D: with micro-niches in which they control chemical, topographical and rheological cues using PRIMO photopatterning system!
Read their Paper.
See how Jorge Ferreira form i3S, Universidade do Porto used PRIMO custom micropatterning technology to control cellular geometry and impose spatial constraints during mitotic progression.
And read his feedback on PRIMO technology.
New scientific paper in which PRIMO was used: In situ photo-patterning of pressure-resistant hydrogel membranes with controlled permeabilities in PEGDA microfluidic channels, Lab Chip, 2018, Advance Article, Jérémy Decock, Mathias Schlenkb and Jean-Baptiste Salmon.
Read it here
List of papers using PRIMO here
2D-STORM image by Abbelight of patterned epithelial cells labelled for Actin on square micropatterns done with PRIMO.
Epithelial cells plated on a grid micropatterned with PRIMO: Actin (green), focal adhesions (red) and nuclear enveloppe (blue).
We witnessed a micrometric solar eclipse over France before the real one happened in the USA! Scale bar = 50µm
Pattern of the day done with PRIMO, Eiffel Tower in Fibronectin and then with MDCK cells.
Thibaut Brunet shared on Twitter a picture of cells plated on micropatterns he generated with PRIMO, during the Physiology summer course at the Marine Biological Laboratory (MBL). Imaged by Nikon USA.
Alvéole développe des outils innovants pour contrôler le microenvironnement sur les supports standards de culture cellulaire en 2D et 3D.
Consultez la liste des conférences et workshops auxquels nous participons ou organisons. Nous serons ravis de vous y présenter PRIMO et de discuter avec vous de vos projets d’applications!
Nos utilisateurs décrivent leurs projets de recherche et nous expliquent pourquoi ils ont choisi d’utiliser PRIMO !