Controlling the topography and biochemistry of cell culture substrates with PRIMO® photopatterning system

In vivo, the cellular microenvironment has a crucial impact on the regulation of cell behavior and functions such as cellular differentiation, proliferation and migration. One of the challenges confronting cell biologists is to mimic this microenvironment in vitro to more efficiently study living cells and model diseases. Here we present PRIMO: a contactless and maskless UV projection system, which allows to control the topography and chemistry of in vitro microenvironments. We first show that PRIMO is a suitable tool to structure photosensitive resists and create molds on which elastomeric solutions (PDMS) can be polymerized. Then we show that the structured PDMS can be specifically functionalized with biomolecules using UV-light structured by PRIMO and a specific photo-initiator (PLPP). Altogether, we demonstrate that PRIMO allows to tailor the cell microenvironment topography through microfabrication and biochemistry through micropatterning.

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Controlling the topography and biochemistry of cell culture substrates with PRIMO® photopatterning system

New Tools for Understanding the Role of the Extracellular Matrix in Cell Morphology: A Combined Photopatterning in Nanotopography Study

In this application note, the differential impact of the chemical and structural components of the Extracellular Matrix (ECM) was investigated and compared to the structure and organization of HeLa cells. Morphology was assessed for cells cultured on micropatterned Fibronectin (FN) protein arrays formed on nanotopographical features. Through this approach, the delineation of the role and importance of each of these extracellular cues in directing cell organization was accomplished. Spatial patterning of ECM proteins has been repeatedly demonstrated to closely control cell and tissue shape and intracellular organization1–5. When FN patterns are formed in the presence of nanoscale physical features, cells will preferentially align to the orientation of the mechanical cues, while the specific transmembrane proteins that involved in cell adhesion will organize based on the FN patterns.

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New Tools for Understanding the Role of the Extracellular Matrix in Cell Morphology: A Combined Photopatterning in Nanotopography Study