Spatially organized and reproducible spheroid formation using PRIMO contactless photopatterning system
In this application note, we show two ways of using PRIMO contactless photopatterning system to make hundreds of very reproducible 3D cell aggregates. A first method consists in growing cells in 3D cuvettes made of non-adherent hydrogel, while the other allows their growth in 3D from a two-dimensional (2D) micropattern. Another limitation of current methods is the difficulty to monitor spheroid evolution or to image them because of their difficult handling. We also show by making a well-organized 3D tumor invasion assay that our techniques allow a perfect organization of experiments in space, thus allowing easier subsequent imaging and data analysis. Altogether, this application note shows that PRIMO can be a powerful tool to produce reproducible 3D cell aggregates, control their shape, and organize them in space for a precise automated data analysis.