Micropatterning of primary neurons with PRIMO
In this application note, we proposed a full micropatterning protocol widely accessible to neuroscientists. We showed the compatibility of our micropatterning protocol with the Banker culture protocol, very commonly used for primary hippocampal neurons. We demonstrated that we can successfully keep neurons in culture for weeks thanks to the PLPP Gel protocol, allowing analysis to be carried out throughout neuronal development. Altogether, PRIMO’s capabilities to easily create multiprotein patterned substrates or print gradients of proteins open a wide range of possibilities in the development of new in vitro assays in Neuroscience.
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