Photopatterning with Primo

WHAT ARE THE ADVANTAGES OF PHOTOPATTERNING WITH PRIMO COMPARE TO OTHER MICROPATTERNING TECHNIQUES?

The PRIMO solution gives researchers complete freedom and full control over their manipulations to optimize the experimental conditions easily. PRIMO allows any shape of protein pattern to be generated rapidly, to precisely align or overlay several proteins (up to 3 without restrictions depending on the proteins used and their interactions) and to control the local density of protein adsorbed to the substrate.
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The PRIMO module

WHAT TYPE OF LASER IS BUILT INTO THE PRIMO UNIT?

The PRIMO unit contains a UV laser operating at 375 nm.
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The dedicated Leonardo software

WHAT ARE THE FEATURES OF THE LEONARDO SOFTWARE?

  • Replication of the same pattern (settings in rows and columns, and adjustment of spacing)
  • Realignment of successive patterns or on microstructures (preview, rotation)
  • Gradient management
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Photopatterning platform and reagents

WHAT EQUIPMENT IS NEEDED TO USE PRIMO?

  • Inverted microscope fitted with focus maintaining system(1):
    • Nikon Ti-E and Ti-2
    • Leica DMi8
    • Olympus IX83
  • Epifluorescence system
  • Motorized X/Y stage
  • Camera
  • Computer
  • Plasma reactor and dedicated pump(2)
(1)Full microscope configuration available on request. (2)Optional depending on experimental conditions.
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