Extracellular matrix stiffness regulates force transmission pathways in multicellular ensembles of human airway smooth muscle cells.
For an airway or a blood vessel to narrow, there must be a connected path that links the smooth muscle (SM) cells with each other, and transmits forces around the organ, causing it to constrict. Currently, we know very little about the mechanisms that regulate force transmission pathways in a multicellular SM ensemble. Here, we used extracellular matrix (ECM) micropatterning to study force transmission in a two-cell ensemble of SM cells. … Read the scientific paper
The cytoskeleton as a smart composite material: A unified pathway linking microtubules, myosin-II filaments and integrin adhesions
The interrelationship between microtubules and the actin cytoskeleton in mechanoregulation of integrin-mediated adhesions is poorly understood. Here, we show that the effects of microtubules on two major types of cell-matrix adhesions, focal adhesions and podosomes, are mediated by KANK family proteins connecting the adhesion protein talin with microtubule tips. Both total microtubule disruption and microtubule uncoupling from adhesions by manipulations with KANKs trigger a massive assembly of myosin-IIA filaments. … Read the scientific paper
A generic widefield topographical and chemical photopatterning method for hydrogels.
Physiologically relevant cell-based models require engineered microenvironments which recapitulate the topographical, biochemical and mechanical properties encountered in vivo. In this context hydrogels are the materials of choice. However, shaping hydrogels at the cellular scale and tuning their chemical properties requires deep investment in custom chemistry and devices while more accessible ones lack a simple structuration and functionalization mean. Here, we show how the most commonly used hydrogels (i.e. Matrigel, Agar, PEG, Polyacrylamide) can be finely structured and spatially functionalized by exploiting oxygen and radical photochemistry together with a widefield patterned UV light illumination. … Read the scientific paper
Adv Biosys, 2018
A new approach to design artificial 3D micro-niches with combined chemical, topographical and rheological cues
The in vitro methods to recapitulate environmental cues around cells are usually optimized to test a specific property of the environment (biochemical nature or the stiffness of the extra cellular matrix (ECM), or nanotopography) for its capability to induce defined cell behaviors (lineage commitment, migration). Approaches that combine different environmental cues in 3D to assess the biological response of cells to the spatial organization of different biophysical and biochemical cues are growingly being developed. … Read the scientific paper
Lab Chip, 2018
In situ photopatterning of pressure-resistant hydrogel membranes with controlled permeabilities in PEGDA microfluidic channels.
We report the fabrication of highly permeable membranes in poly(ethylene glycol) diacrylate (PEGDA) channels, for investigating ultra- or micro-filtration, at the microfluidic scale. More precisely, we used a maskless UV projection setup to photopattern PEG-based hydrogel membranes on a large scale (mm–cm), and with a spatial resolution of a few microns. We show that these membranes can withstand trans-membrane pressure drops of up to 7 bar without any leakage, thanks to the strong anchoring of the hydrogel to the channel walls. … Read the scientific paper
Collagen assembly and turnover imaged with a CRISPR-Cas9 engineered Dendra2 tag
Electron microscopy has been the gold standard for studying collagen networks but dynamic information on how cells synthesise the networks has been lacking. Live imaging methods have been unable to distinguish newly-synthesised fibrils from pre-existing fibrils and intracellular collagen. Here, we tagged endogenous collagen-I using CRISPR-Cas9 with photoswitchable Dendra2 and demonstrate live cells synthesising, migrating on, and interacting with, collagen fibrils. … Read the scientific paper
Hum Mol Genet. 2016
Altered microtubule dynamics and vesicular transport in mouse and human MeCP2- deficient astrocytes.
Rett syndrome (RTT) is a rare X-linked neurodevelopmental disorder, characterized by normal post-natal development followed by a sudden deceleration in brain growth with progressive loss of acquired motor and language skills, stereotypic hand movements and severe cognitive impairment. Mutations in the methyl-CpG-binding protein 2 (MECP2) cause more than 95% of classic cases. … Read the scientific paper
Adv Mater. 2016
Multiprotein Printing by Light-Induced Molecular Adsorption.
Light-induced molecular adsorption of proteins (LIMAP) allows for quantitative sub-micrometer-resolution printing of multiple biomolecules. Surface-bound gradients are patterned within minutes over an entire glass cover-slip. LIMAP is used to perform selective immuno-assays, to dynamically control the adhesion of individual cells, and to achieve hierarchical co-cultures instrumental for tissue engineering. Read the scientific paper