Your project of experiment with PRIMO
“Protein micropatterning represents an excellent tool to probe the behavior and functions of cellular systems. PRIMO is specially suited for our experiments, in which the cell-substrate interaction needs to be precisely adjusted both throughout the substrates and in time, in order to control the dynamic behaviour of cell monolayers.”
I’ve mainly used (and use) photomasks and, although they are very useful and robust, they of course lack adjustability. Especially in trial stages, photomasks are not convenient.
In addition, the fact that the masks need to be in direct contact with the substrates is not ideal as the surface functionalization can be damaged.
Finally, mask-based photopattening is always “B/W”, meaning that it is not possible to quantitatively control protein density within a pattern. Despite laser-rasterized designs would allow so, I think it does not meet the requirements for cell-based studies, in terms of precision or physiological relevance.
PRIMO allows patterning with very high resolution on a wide range of substrates, ceramic and polymeric, flat and microstructured, with arbitrary patterns of light, with intensities that can span the whole grey scale.
Remarkably, this enables the preparation of patterns with quantitatively different protein density, and the preparation of surface-bound fine protein gradients.
Furthermore, the possibility to modify the protein patterns in situ, the so-called dynamical patterning, opens a wide spectrum of experimental possibilities.