Your project of experiment with PRIMO

“Protein micropatterning represents an excellent tool to probe the behavior and functions of cellular systems. PRIMO is specially suited for our experiments, in which the cell-substrate interaction needs to be precisely adjusted both throughout the substrates and in time, in order to control the dynamic behaviour of cell monolayers.”

cell-adhesion
Pattern used to place cells towards two adhesion options. Courtesy of A. Roux' Lab.

Madin-Darby Canine Kidney epithelial cells onto patterns of fibronectin/fibrinogen. Cells attach preferentially onto regions with higher protein density. Courtesy of Aurelien Roux’ Laboratory.
cell-gradient-pattern
Pattern used to place cells towards a gradient. Courtesy of A. Roux' Lab.

Madin-Darby Canine Kidney epithelial cells onto patterns of fibronectin/fibrinogen. Cells attach preferentially onto regions with higher protein density. Courtesy of Aurelien Roux’ Laboratory.

Why PRIMO

I’ve mainly used (and use) photomasks and, although they are very useful and robust, they of course lack adjustability. Especially in trial stages, photomasks are not convenient.

In addition, the fact that the masks need to be in direct contact with the substrates is not ideal as the surface functionalization can be damaged.

Finally, mask-based photopattening is always “B/W”, meaning that it is not possible to quantitatively control protein density within a pattern. Despite laser-rasterized designs would allow so, I think it does not meet the requirements for cell-based studies, in terms of precision or physiological relevance.

PRIMO allows patterning with very high resolution on a wide range of substrates, ceramic and polymeric, flat and microstructured, with arbitrary patterns of light, with intensities that can span the whole grey scale.

Remarkably, this enables the preparation of patterns with quantitatively different protein density, and the preparation of surface-bound fine protein gradients.

Furthermore, the possibility to modify the protein patterns in situ, the so-called dynamical patterning, opens a wide spectrum of experimental possibilities.

Little extra which makes all the difference

Performances :

PRIMO performance was very satisfactory. The ease-of-use of both the device and LEONARDO software help to achieve great results in a short time. Of course, depending on the experimental requirements, the surface functionalization, illumination time, light dose and protein concentration have to be adjusted and optimized. PRIMO did not fail when the settings were suitable.

Support provided by Alvéole team:

It couldn’t have been better. Support from the very beginning, rapid response to our queries, and 100% efficient solutions.

“We are working on the generation of 3D cellular microenvironments to reproduce Hematopoietic Niches. PRIMO will be used to generate 3D photo-polymerized microenvironments and to pattern them to localize different cell populations involved in the hematopoiesis.”

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“We are currently particularly interested in determining the role of the biophysical environment in the establishment of apico-basal polarity in mammary gland cells and in liver cells. The use of PRIMO in this context proved absolutely essential since it allowed us to create artificial microniches in 3D where we could control up to 150 combinations of environmental cues.”

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“Our aim is to develop in vitro experimentation to decipher guiding mechanisms involved in vivo. PRIMO technology is particularly adapted to design in vitro microdevices patterned with controlled patches of the signaling proteins relevant for white blood cell migration.”

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“My research focuses on the mechanical aspects of cell division. With the PRIMO system we wanted to control cellular geometry to impose spatial constraints during mitotic progression.”

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“We are interested in imaging subcellular localization of certain cell-surface receptors and check whether they colocalize with focal-adhesion complexes. For this purpose, we are interested in making different types of patterns of Fibronectin with subcellular dimensions.”

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“My research project aims at unravelling how a T cell switches from a fast migratory state to a stationary state upon activation. To do so, I perform live cell imaging of T cells migrating inside micro-fabricated channels coated with activating molecules. However, with this approach, I do not control when and where a T cell encounters the activating molecules.”

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Controlling
the cellular
microenvironment

Alvéole has developed innovative solutions adapted to all standard cell culture substrates, rigid or soft, in 2D or 3D.

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Resource Center

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Testimonials

Our users describe their research projects and explain why they chose to use PRIMO!

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