Your project of experiment with PRIMO

“My research project aims at unravelling how a T cell switches from a fast migratory state to a stationary state upon activation. To do so, I perform live cell imaging of T cells migrating inside micro-fabricated channels coated with activating molecules. However, with this approach, I do not control when and where a T cell encounters the activating molecules.

The use of PRIMO allows me to print a “migratory zone” and an “activation zone” along micro-channels to visualize this transition.”

microchannel pattern
Single lymphocyte (cell nucleus staining with Hoechst) migrating in a microchannel patterned with a migratory zone (fibronectin) and an activating zone (anti-CD3 Abs).

Why PRIMO

I have been using UV-based micropatterning technique in the past. Although this technique is powerful to pattern several coverslips at a time, it does not allow multiple protein patterning and requires the design and fabrication of a new chromium mask for every new pattern that need to be tested.

I chose to use PRIMO for its capacity to print proteins on 3D surfaces and because it enables me to fine-tune the printing area on a per experiment basis.

Little extra which makes all the difference

Performances :

PRIMO has almost no limitations in terms of surface chemistry, surfaces to be printed and is fast once the system has been optimized/adjusted to your requirement.

Support provided by Alvéole team:

The team has been very supportive and helpful even for people working “down under” such as us! They provided feedbacks and protocols to optimize our experiments. I also appreciated their willingness to stay in touch with us and to create a PRIMO community.

“We are working on the generation of 3D cellular microenvironments to reproduce Hematopoietic Niches. PRIMO will be used to generate 3D photo-polymerized microenvironments and to pattern them to localize different cell populations involved in the hematopoiesis.”

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“We are currently particularly interested in determining the role of the biophysical environment in the establishment of apico-basal polarity in mammary gland cells and in liver cells. The use of PRIMO in this context proved absolutely essential since it allowed us to create artificial microniches in 3D where we could control up to 150 combinations of environmental cues.”

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“Our aim is to develop in vitro experimentation to decipher guiding mechanisms involved in vivo. PRIMO technology is particularly adapted to design in vitro microdevices patterned with controlled patches of the signaling proteins relevant for white blood cell migration.”

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“My research focuses on the mechanical aspects of cell division. With the PRIMO system we wanted to control cellular geometry to impose spatial constraints during mitotic progression.”

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“We are interested in imaging subcellular localization of certain cell-surface receptors and check whether they colocalize with focal-adhesion complexes. For this purpose, we are interested in making different types of patterns of Fibronectin with subcellular dimensions.”

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“Protein micropatterning represents an excellent tool to probe the behavior and functions of cellular systems. PRIMO is specially suited for our experiments, in which the cell-substrate interaction needs to be precisely adjusted both throughout the substrates and in time, in order to control the dynamic behaviour of cell monolayers.”

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Controlling
the cellular
microenvironment

Alvéole has developed innovative solutions adapted to all standard cell culture substrates, rigid or soft, in 2D or 3D.

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Resource Center

Our team gives you all its tips you need to carry out your experimental manipulations and go even further!

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Testimonials

Our users describe their research projects and explain why they chose to use PRIMO!

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