Your project of experiment with PRIMO
“My research project aims at unravelling how a T cell switches from a fast migratory state to a stationary state upon activation. To do so, I perform live cell imaging of T cells migrating inside micro-fabricated channels coated with activating molecules. However, with this approach, I do not control when and where a T cell encounters the activating molecules.
The use of PRIMO allows me to print a “migratory zone” and an “activation zone” along micro-channels to visualize this transition.”
Why PRIMO
I have been using UV-based micropatterning technique in the past. Although this technique is powerful to pattern several coverslips at a time, it does not allow multiple protein patterning and requires the design and fabrication of a new chromium mask for every new pattern that need to be tested.
I chose to use PRIMO for its capacity to print proteins on 3D surfaces and because it enables me to fine-tune the printing area on a per experiment basis.
Little extra which makes all the difference
Performances :
PRIMO has almost no limitations in terms of surface chemistry, surfaces to be printed and is fast once the system has been optimized/adjusted to your requirement.
Support provided by Alvéole team:
The team has been very supportive and helpful even for people working “down under” such as us! They provided feedbacks and protocols to optimize our experiments. I also appreciated their willingness to stay in touch with us and to create a PRIMO community.
