Your project of experiment with PRIMO
“My research project aims at unravelling how a T cell switches from a fast migratory state to a stationary state upon activation. To do so, I perform live cell imaging of T cells migrating inside micro-fabricated channels coated with activating molecules. However, with this approach, I do not control when and where a T cell encounters the activating molecules.
The use of PRIMO allows me to print a “migratory zone” and an “activation zone” along micro-channels to visualize this transition.”
I have been using UV-based micropatterning technique in the past. Although this technique is powerful to pattern several coverslips at a time, it does not allow multiple protein patterning and requires the design and fabrication of a new chromium mask for every new pattern that need to be tested.
I chose to use PRIMO for its capacity to print proteins on 3D surfaces and because it enables me to fine-tune the printing area on a per experiment basis.
Little extra which makes all the difference
PRIMO has almost no limitations in terms of surface chemistry, surfaces to be printed and is fast once the system has been optimized/adjusted to your requirement.
Support provided by Alvéole team:
The team has been very supportive and helpful even for people working “down under” such as us! They provided feedbacks and protocols to optimize our experiments. I also appreciated their willingness to stay in touch with us and to create a PRIMO community.