Your project of experiment with PRIMO
“We are interested in imaging subcellular localization of certain cell-surface receptors and check whether they colocalize with focal-adhesion complexes. For this purpose, we are interested in making different types of patterns of Fibronectin with subcellular dimensions.”
Before PRIMO, we used PDMS based microcontact printing of proteins mostly in PBS on various substrates. But the results were poor prints, patterns dependent on available masters and proteins printed as multilayers instead of monolayer.
Our lab uses photo-responsive ligands to make dynamic biomaterials, so in general PRIMO was a useful tool for most of our members.
In particular, it suited my requirement of making patterns with different dimensions and features and being able to immobilize any protein of interest onto it. Multiple protein patterns would also be something of interest for the future.
Little extra which makes all the difference
Calibration before the start of an experiment is very easy and quick. The instrument performs extremely well.
Support provided by Alvéole team:
The team provided a good and reactive support. People are available to answer question. And last but not least, they are very friendly people.