Your project of experiment with PRIMO

“We are interested in imaging subcellular localization of certain cell-surface receptors and check whether they colocalize with focal-adhesion complexes. For this purpose, we are interested in making different types of patterns of Fibronectin with subcellular dimensions.”

Rhodamine patterns
SKBR3 cells spread over Fibronectin-Rhodamine patterns.

Why PRIMO

Before PRIMO, we used PDMS based microcontact printing of proteins mostly in PBS on various substrates. But the results were poor prints, patterns dependent on available masters and proteins printed as multilayers instead of monolayer.

Our lab uses photo-responsive ligands to make dynamic biomaterials, so in general PRIMO was a useful tool for most of our members.

In particular, it suited my requirement of making patterns with different dimensions and features and being able to immobilize any protein of interest onto it. Multiple protein patterns would also be something of interest for the future.

Little extra which makes all the difference

Performances :

Calibration before the start of an experiment is very easy and quick. The instrument performs extremely well.

Support provided by Alvéole team:

The team provided a good and reactive support. People are available to answer question. And last but not least, they are very friendly people.

“We are working on the generation of 3D cellular microenvironments to reproduce Hematopoietic Niches. PRIMO will be used to generate 3D photo-polymerized microenvironments and to pattern them to localize different cell populations involved in the hematopoiesis.”

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“We are currently particularly interested in determining the role of the biophysical environment in the establishment of apico-basal polarity in mammary gland cells and in liver cells. The use of PRIMO in this context proved absolutely essential since it allowed us to create artificial microniches in 3D where we could control up to 150 combinations of environmental cues.”

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“Our aim is to develop in vitro experimentation to decipher guiding mechanisms involved in vivo. PRIMO technology is particularly adapted to design in vitro microdevices patterned with controlled patches of the signaling proteins relevant for white blood cell migration.”

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“My research focuses on the mechanical aspects of cell division. With the PRIMO system we wanted to control cellular geometry to impose spatial constraints during mitotic progression.”

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“My research project aims at unravelling how a T cell switches from a fast migratory state to a stationary state upon activation. To do so, I perform live cell imaging of T cells migrating inside micro-fabricated channels coated with activating molecules. However, with this approach, I do not control when and where a T cell encounters the activating molecules.”

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“Protein micropatterning represents an excellent tool to probe the behavior and functions of cellular systems. PRIMO is specially suited for our experiments, in which the cell-substrate interaction needs to be precisely adjusted both throughout the substrates and in time, in order to control the dynamic behaviour of cell monolayers.”

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Controlling
the cellular
microenvironment

Alvéole has developed innovative solutions adapted to all standard cell culture substrates, rigid or soft, in 2D or 3D.

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Resource Center

Our team gives you all its tips you need to carry out your experimental manipulations and go even further!

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Testimonials

Our users describe their research projects and explain why they chose to use PRIMO!

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