Your project of experiment with PRIMO

“The team is studying the recruitment mechanisms of white blood during an immune response. The complex orchestration of white blood cells traffic between blood/lymph systems, lymph nodes and inflammation zones relies on sophisticated but still poorly understood signaling routes that guide cells toward precise targets.
Our aim is to develop in vitro experimentation to decipher guiding mechanisms involved in vivo.”

PRIMO technology is particularly adapted to design in vitro microdevices patterned with controlled patches of the signaling proteins relevant for white blood cell migration.

lymphocytes-adhesion-strips
Primary human T lymphocytes on specific adhesion strips.

Why PRIMO

“We previously used PDMS microstamping but the amount of deposited proteins was uncontrolled and we had difficulties to print gradients.
We chose to use PRIMO because it allows to print gradients as well as a combination of several proteins”

Little extra which makes all the difference

Performances :

The optical printing system of PRIMO is highly performant and user-friendly. The best is still to come.

Support provided by Alvéole team:

Totally satisfying”

“We are working on the generation of 3D cellular microenvironments to reproduce Hematopoietic Niches. PRIMO will be used to generate 3D photo-polymerized microenvironments and to pattern them to localize different cell populations involved in the hematopoiesis.”

Read the testimonial

“We are currently particularly interested in determining the role of the biophysical environment in the establishment of apico-basal polarity in mammary gland cells and in liver cells. The use of PRIMO in this context proved absolutely essential since it allowed us to create artificial microniches in 3D where we could control up to 150 combinations of environmental cues.”

Read the testimonial

“My research focuses on the mechanical aspects of cell division. With the PRIMO system we wanted to control cellular geometry to impose spatial constraints during mitotic progression.”

Read the testimonial

“We are interested in imaging subcellular localization of certain cell-surface receptors and check whether they colocalize with focal-adhesion complexes. For this purpose, we are interested in making different types of patterns of Fibronectin with subcellular dimensions.”

Read the testimonial

“My research project aims at unravelling how a T cell switches from a fast migratory state to a stationary state upon activation. To do so, I perform live cell imaging of T cells migrating inside micro-fabricated channels coated with activating molecules. However, with this approach, I do not control when and where a T cell encounters the activating molecules.”

Read the testimonial

“Protein micropatterning represents an excellent tool to probe the behavior and functions of cellular systems. PRIMO is specially suited for our experiments, in which the cell-substrate interaction needs to be precisely adjusted both throughout the substrates and in time, in order to control the dynamic behaviour of cell monolayers.”

Read the testimonial

Controlling
the cellular
microenvironment

Alvéole has developed innovative solutions adapted to all standard cell culture substrates, rigid or soft, in 2D or 3D.

Learn more

Resource Center

Our team gives you all its tips you need to carry out your experimental manipulations and go even further!

Learn more

Testimonials

Our users describe their research projects and explain why they chose to use PRIMO!

Read the testimonials