Your project of experiment with PRIMO

“We are working on the generation of 3D cellular microenvironments to reproduce Hematopoietic Niches. PRIMO will be used to generate 3D photo-polymerized microenvironments and to pattern them to localize different cell populations involved in the hematopoiesis.”

cells-Fibronectin
RPE1 cells on an Eiffel Tower micropattern generated with PRIMO using Fibronectin and Fibrinogen-Alexa647.

Why PRIMO

Before PRIMO, we used deep UV photolithography through a chromium mask or pulsed UV laser writing to locally remove the PEG adsorbed on the surface of the sample. However, the quality of the pegylation has to be high, and sometimes some bad contact between the mask and the PEG surface may generate blurry patterns or defects on the passivation.

We initially chose to use PRIMO because it allows sequential multi patterning and makes gradient patterning possible. It works with a broad range of scale and its design creation is limitless.

Little extra which makes all the difference

Performances:

The technology is reproducible and robust after good practical and highly performant to prototype designs or protein combinations.

Support provided by Alvéole team:

Technical support as well as scientific support are very nice for installation and beginning of new project.

Testimonial completed with Benoit Vianay, CEA Engineer at the Physics of cytoskeleton & Morphogenesis lab (Cytomorpholab)

“We are currently particularly interested in determining the role of the biophysical environment in the establishment of apico-basal polarity in mammary gland cells and in liver cells. The use of PRIMO in this context proved absolutely essential since it allowed us to create artificial microniches in 3D where we could control up to 150 combinations of environmental cues.”

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“Our aim is to develop in vitro experimentation to decipher guiding mechanisms involved in vivo. PRIMO technology is particularly adapted to design in vitro microdevices patterned with controlled patches of the signaling proteins relevant for white blood cell migration.”

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“My research focuses on the mechanical aspects of cell division. With the PRIMO system we wanted to control cellular geometry to impose spatial constraints during mitotic progression.”

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“We are interested in imaging subcellular localization of certain cell-surface receptors and check whether they colocalize with focal-adhesion complexes. For this purpose, we are interested in making different types of patterns of Fibronectin with subcellular dimensions.”

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“My research project aims at unravelling how a T cell switches from a fast migratory state to a stationary state upon activation. To do so, I perform live cell imaging of T cells migrating inside micro-fabricated channels coated with activating molecules. However, with this approach, I do not control when and where a T cell encounters the activating molecules.”

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“Protein micropatterning represents an excellent tool to probe the behavior and functions of cellular systems. PRIMO is specially suited for our experiments, in which the cell-substrate interaction needs to be precisely adjusted both throughout the substrates and in time, in order to control the dynamic behaviour of cell monolayers.”

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Controlling
the cellular
microenvironment

Alvéole has developed innovative solutions adapted to all standard cell culture substrates, rigid or soft, in 2D or 3D.

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Resource Center

Our team gives you all its tips you need to carry out your experimental manipulations and go even further!

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Testimonials

Our users describe their research projects and explain why they chose to use PRIMO!

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