Key steps of protein micropatterning with PRIMO

PRIMO photopatterning module allows to project on the substrate any image you want in order to create a protein micropattern. The combined action of UV (λ=375nm) and PLPP degrades the anti-fouling coating, in only a few seconds. The protein can then be added and will adsorb on the illuminated areas only.

PRIMO: a versatile micropatterning technology

PRIMO is compatible with all standard cell culture substrates (glass coverslips, plastic Petri dishes) and also with soft substrates frequently used for cell culture (PDMS, polyacrylamide gel).

Standard substrates:

glass coverslips, plastic Petri dishes, etc.

Epifluorescence microscopy
Epifluorescence microscopy image of 2 µm horizontal lines of Protein A-488 on glass.
Fibroblasts plated on fibronectin micropatterns
Fibroblasts plated on fibronectin micropatterns on a glass coverslip (after 2 weeks).

Soft substrates:

PDMS, polyacrylamide gel, hydrogels

Epifluorescence microscopy image
Epifluorescence microscopy image of 1.5 µm dots (spaced by 1.5 µm) of ProteinA-488 on PDMS.
Functionalized hydrogel: "In overlay: radial fluorescence intensity profile (red) of the FITC signal. Right panel: cells adhering on the corresponding pattern with fibronectin at day 0." A. Pasturel et al., BioRxiv, 2018

Micropatterning of a wide range of molecules

More than 10 proteins are used daily by our users, including:
Fibrinogen-488, Fibrinogen-647, Fibronectin, GFP, Neutravidin-488, Neutravidin-647, PLL-PEG-Biotin, Protein A-647, Streptavidin, as well as primary and secondary antibodies.

Do you have a question about your project of experiment with PRIMO?

Our research and application development team can help you set up or optimize your experimental protocols!

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