This is the 3/4 videos from the 2nd edition of our Virtual Symposium, featuring the last publications and results from our users in cell biology and mechanobiology.

PRIMO bioengineering technology allows the reconstruction of functional intrahepatic bile ducts with predefined geometry

Building bile ducts is a challenging task for liver tissue engineering, in particular the reconstruction of the intrahepatic biliary tree consisting of 10-100µm ducts. Although several methods were used to address such a challenge, the resulting structures either had luminal diameter corresponding to the extrahepatic part of the tree or had unpredictable geometry [1]. We therefore developed a biocompatible stereolithographic approach to encapsulate cholangiocytes, the biliary epithelial cells in 3D structured hydrogels mimicking the branched biliary network [2]. This strategy allowed us to produce functional Biliary Structures by PhotoPolymerization (BSPPs) with a predefined geometry and with a final luminal diameter at the intrahepatic duct scale [3].

[1]Lewis, P. L. et al. Sci. Rep. 8, 12220 (2018).
[2] Ma, X. et al. Proc. Natl. Acad. Sci. 113, 2206 (2016).
[3] Mazari-Arrighi, E. et al. Biomaterials. 279, 121207 (2021).
This work received the financial support of the iLite RHU program (grant ANR ANR-16-RHUS-0005)

Presented by: Elsa Mazari-Arrighi, who holds a PhD in biophysics from Paris-Sud University and is specialized in development of 3D architectures for cell biology.  Since 2017, she is a postdoctoral researcher at Hospital St Louis in a joint research unit (U976 Stem Cell Technologies, Inserm/ Université Paris Diderot) and she focuses on the development of 3D bioprinting approaches to reconstruct functional tissues.

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