Alvéole and Leica Microsystems conclude a partnership

Like many of you, we are trying to adapt to the situation, to stay in touch and to keep talking about science! Our R&D team invites you to set small group online discussions on topics related to PRIMO photopatterning system micropatterning and cellular microenvironment.

Find more info to set a call with our team.

Ask your questions live to Dr Leeya Engel during our sponsored Nature Research webcast on « Micropatterning on EM grids: A strategy for improving cell cryo-ET workflow » > Controlling cell positioning within EM grids. Attend our webinar.

Here comes another paper using PRIMO contactless micropatterning on TEM Grids! « Tailoring cryo-electron microscopy grids by photo-micropatterning for in-cell structural studies », M. Toro-Nahuelpan et al, BioRxiv, 2019. From Julia Mahamid’s team at EMBL.

Read the paper.

« Substrate area confinement is a key determinant of cell velocity in collective migration », Mohammed et al, Nature Physics, 2019. –> They used micropatterning to mimic the spatial confinement experienced by follower cells within epithelial tissues.

Access the research paper.

Aurélien Pasturel (PhD candidate, CNRS, Alvéole) presents us his work: « Tailoring 3D cell culture templates with common hydrogels », A Pasturel, BioRxiv, 2018

Watch the scientific video.

What’s the common point between a laboratory mouse and a Petri dish? See the answer in video.

Giant cells moving on a building and adapting to obstacles. We are amazed by the CytoMorphoLab team’s creativity and how they used PRIMO bioengineering technology to miniaturise a building to the size of cells!

Find more details about the whole project on their website & make sure you watch THE VIDEO!

Are you interested in understanding the role of the extracellular matrix in cell morphology? This application note is for you.

Check how we worked with Nanosurface Biomedical to micropattern their microstructured substrate.

Access the Application Note.

Using quantitative antibodies micropatterning with PRIMO and RICM imaging, the team studied haptotaxis of T lymphocytes.

See all our users’ scientific papers.

Did you miss our live webinar presented by Dr. Manuel Théry and Dr. Harikrishnan Parameswaran? They explained how PRIMO allows to work with controlled and reproducible microenvironments in vitro to more efficiently study living cells and model diseases.

Register and watch the replay on Nature dedicated webpage.

Last week we were at The University of Geneva to instal PRIMO after a successful demo!

Pau Guillamat from Aurélien Roux’ lab uses PRIMO contactess and maskless micropatterning to study epithelial dynamics.

Check some of his previous work with PRIMO.

The team who loaned a PRIMO for this year Physiology Summer Course at the Marine Biological Laboratory made quite an experiment!

To study cell junctions under curvature, they patterned MDCK cells on a spiral fibronectin pattern.

Check the video.

« Controlling the topography and biochemistry of cell culture substrates with PRIMO »

One of the challenges confronting cell biologists is to mimic the micro-environment in vitro to more efficiently study living cells. (…) PRIMO allows to tailor cell microenvironment topography via microfabrication and biochemistry via micropatterning…

Read the application note

When you offer cells a choice… Yes they go for the higher protein density! Experiment conducted by Pau Guillamat from Aurelien Roux’ lab – University of Geneva.

Watch the video of cells migrating towards two different protein densities in Pau Guillamat’s testimonial.

Virgile Viasnoff‘s lab at the Mechanobiology Institute – National University of Singapore is going further in controlling cell microenvironment in 3D: with micro-niches in which they control chemical, topographical and rheological cues using PRIMO photopatterning system! 

Read their Paper.

See how Jorge Ferreira form i3S, Universidade do Porto used PRIMO custom micropatterning technology to control cellular geometry and impose spatial constraints during mitotic progression.

And read his feedback on PRIMO technology.

New scientific paper in which PRIMO was used:  In situ photo-patterning of pressure-resistant hydrogel membranes with controlled permeabilities in PEGDA microfluidic channels, Lab Chip, 2018, Advance Article, Jérémy Decock, Mathias Schlenkb and Jean-Baptiste Salmon.

Read it here

List of papers using PRIMO here

2D-STORM image by Abbelight of patterned epithelial cells labelled for Actin on square micropatterns done with PRIMO.

Epithelial cells plated on a grid micropatterned with PRIMO: Actin (green), focal adhesions (red) and nuclear enveloppe (blue).

We witnessed a micrometric solar eclipse over France before the real one happened in the USA! Scale bar = 50µm

Pattern of the day done with PRIMO, Eiffel Tower in Fibronectin and then with MDCK cells.

Thibaut Brunet shared on Twitter a picture of cells plated on micropatterns he generated with PRIMO, during the Physiology summer course at the Marine Biological Laboratory (MBL). Imaged by Nikon USA.