#1 Drawing micropatterns for PRIMO

The PRIMO solution can create with high flexibility any protein micropattern on all standard cell culture substrates. An image of the micropattern (no restriction on shape except for the minimal resolution of 1.2 μm) must first be loaded into Leonardo software, which will send it to the PRIMO module and project it in UV light through the microscope objective onto the
substrate. Two file formats can be used with Leonardo software:

  • .tif format: drawing in pixels (ratio is indicated in the Leonardo calibration parameters)
  • .pdf format: drawing in millimeters (actual size of the pattern)

The video presents how you can draw patterns and gradients for PRIMO with the Inkscape
drawing program.

Watch the other tutorials

#2 Preparing your substrates

#3 Switching the PRIMO setup on

#4 Starting the Leonardo software and Calibrating the system

#5 Setting the tube lens position: whenever changing microscope objective / type of substrate

#6 Photopatterning with Leonardo software

#7 Seeding cells on your protein micropatterns

#2 Preparing your substrates

#3 Switching the PRIMO setup on

#4 Starting the Leonardo software and Calibrating the system

#5 Setting the tube lens position: whenever changing microscope objective / type of substrate

#6 Photopatterning with Leonardo software

#7 Seeding cells on your protein micropatterns