#9 Seeding cells on your protein micropatterns

Once the protein patterns have been made with PRIMO, the cells can be seeded and will adhere only to the protein patterns. The stability of cell patterns depends on the substrate used and cell type seeded. Under certain conditions, cells can adhere to protein patterns for up to 30 days.

Watch the other tutorials

#1 Drawing micropatterns for PRIMO

#2 Substrates passivation

#3 NEW: Preparation of TEM grids for micropatterning with PRIMO, passivation

#4 Starting the Leonardo software and Calibrating the system

#5 Setting the tube lens position

#6 Photopatterning with Leonardo software

#7 Protein micropatterning with PLPP Gel

#8 Micropatterns automatic positioning on TEM Grids (or other structures)

#1 Drawing micropatterns for PRIMO

#2 Substrates passivation

#3 NEW: Preparation of TEM grids for micropatterning with PRIMO, passivation

#4 Starting the Leonardo software and Calibrating the system

#5 Setting the tube lens position

#6 Photopatterning with Leonardo software

#7 Protein micropatterning with PLPP Gel

#8 Micropatterns automatic positioning on TEM Grids (or other structures)