Your project of experiment with PRIMO
“My research work consists in understanding how microenvironmental cues inﬂuence, guide and shape cell-cell contacts. We are currently particularly interested in determining the role of the biophysical environment in the establishment of apico-basal polarity in mammary gland cells and in liver cells.
The use of PRIMO in this context proved absolutely essential since it allowed us to create artificial microniches in 3D where we could control up to 150 combinations of environmental cues.
By printing our protein of choice and microfabricating intricate structures under the microscope, we are gradually uncovering how the geometrical and biophysical parameters drive the elongation of lumen into tubes in the context of hepatic development and diseases.”
Before using PRIMO I had a couple trials with protein microstamping and UV printing using masks. These techniques work well for ﬂat 2d surfaces. However, our goal was really to perform 3D microniches with texture. In this all the trials we had with the existing solution were non-conclusive.
PRIMO IS A SUPERB TOOL OF CHOICE IF YOU WANT TO:
- Rapidly prototype different patterns and test which one works best for your cells. You do not need any mask of microfabrication. It really helps “creativity on the spot”.
- Perform mutl-protein printing. PRIMO can reach an impressive ~500nm precision in aligning patterns of 2 diﬀerent proteins. The cross talk is really minimal. We use this approach to extensively study the combined role of Extra-cellular matrix proteins and E-cadherins.
- Print proteins on 3D substrate. This was the original challenge when we received our PRIMO. We had a couple grad students working with the PRIMO team and we now have superb printing capabilities in microwell, curved surfaces with many diﬀerent kinds of proteins and hydrogel.
Little extra which makes all the difference
PRIMO is unique in its capabilities, and for us it is the perfect tool. Submicron-resolution, ﬂexibility and versatility in terms of the proteins you can deal with. It is a very reliable tool to print proteins on surface.
Support provided by Alvéole team:
We were among the ﬁrst to use PRIMO, even as beta testers and the interaction were incredibly fruitful and eﬃcient. After we got the ﬁnal version of the product, the interactions remained the same. We organized a PRIMO workshop in the lab and I could see how professional and passionate the Alvéole team is. They just love their instrument and they want it to work for you and that you love it too.